PMC

HAP binding assay result shows that SNGF at a concentration of 40 μg/ml reduces the specific radioligand binding of the control (17 μ - E₂) to the ER by 50 per cent.

MTT assay shows proliferative effect of SNGF on MCF-7 cells at a concentration of 40 μg/ml concentration and an inhibition of cell proliferation at higher concentrations (80-320 μg/ml). The MCF-7 cells were treated with SNGF at concentrations ranging from 20-320 μg/ml. Values are means ± SE of 5 replicates.

Photomicrographs of Heamatoxylin and Eosin (H&E) stained sections of ovariectomized Swiss albino mice. (a) Water vehicle (control); (b & c) treatment with 100 mg/kg of body wt of SNGF; (b) arrow shows increase in luminal epithelial cell height compared to control; (c) increase in gland number in response to SNGF (10X magnification).

Effect of SNGF on uterine wet wt to body wt ratio of ovariectomized Swiss albino mice. The result shows a 150 per cent increase in uterine wet wt in response to 100 mg/kg concentration of SNGF and a 283 per cent increase in response to positive E₂ (P<0.001). Higher concentrations (250, 500 mg/kg body wt/day) of SNGF did not produce a significant increase in uterine wet wt compared to control. Values are means ± SE of 5 replicates. P***<0.001 compared to control.

Effect of SNGF on ovariectomized mouse uterine epithelial cells. Water fed ovariectomized mice served as control; treatment group mice were fed with 100, 250 & 500 mg/kg body wt of SNGF. They were sacrificed after 7 days of treatment and the uteri were collected. Result indicated an increase in uterine cell height of animals treated with 100 mg/kg SNGF which is 30.8 per cent more than the control. Values represent the mean ± SE values obtained from 5 animals per treatment. P**<0.01, P**<0.001 compared to control.