PMC

Localization of A.t 3-ketoacyl-CoA thiolase wt (A) and equipped with the PTS1 SKL (B) in P. tricornutum; (C) Pt 3-ketoacyl-CoA thiolase upon deletion of its C-terminal tripeptide SSL; (D) GFP equipped with PTS2 (RLQVVLGHL) N-terminal and (E) PTS1 (SKL) C-terminal. PAF, plastid autofluorescence; GFP, GFP fluorescence. Scalebars represent 10 µm.

(A) GFP-catalase, (B) GFP-long chain acyl-CoA ligase, (C) GFP-trans-2-enoyl-CoA reductase, (D) GFP-malate synthase, (E) GFP-3-ketoacyl-CoA thiolase, (F) Pex10-GFP and Pex3-GFP (G). All fusion proteins localize in punctate structures as indicated by the GFP fluorescence (green) next to the complex plastid (red), which is visualized due to the chlorophyll autofluorescence (red). PAF, plastid autofluorescence; GFP, GFP fluorescence. Scalebars represent 10 µm.

(A) Ultrathin section of P. tricornutum expressing Pex10-GFP in Epon without antibody labeling. The boxed area is shown in (B) at higher magnification and illustrates two peroxisomes in proximity to the nucleus, the golgi and the plastid. (C) Immuno labeling of Pex10-GFP in a dividing P. tricornutum cell. (D) higher magnification. The 20 nm gold particle, coupled to the secondary antibody is visible within the peroxisome (arrow head), which is surrounded by a lipid bilayer. CW, cell wall; G, golgi apparatus; Mt, mitochondrium; Ne, nuclear envelope; Nu, nucleus; P, peroxisome; Pl, plastid; V, vacuole; arrow head, 20 nm gold. Scalebars represent 2 µm (A, C), 500 nm (B) and 200 nm (D).