PMC

Mutation overlap. Cases in which a single mutation was detected are depicted in black, whereas those with multiple mutations are depicted in red.

Spectrum of BRAF mutations. Relative frequencies of different BRAF mutations are expressed as the fraction of all BRAF mutations detected in melanoma (n = 73), thyroid (n = 172), and colon (n = 12) cancers.

Mutation frequencies for various genes. Mutation frequencies are displayed by gene as the percentage of total cases tested, for urothelial (n = 85), colorectal (n = 236), endometrioid (n = 90), melanoma (n = 164), and thyroid (n = 245) cancers.

Sensitivity of mass-spectrometry–based mutation detection. A: Specimen with 60% KIT K642E mutant allele frequency was diluted twofold into wild-type DNA, with 10 ng total DNA in each dilution. B: The amount of input DNA was titrated from 20 ng to 0.312 ng. Mutant and wild-type (wt) alleles are indicated by arrows.