PMC

(A) High-magnification image of a spiny dendrite of a CA1 hippocampal pyramidal cell formed from the red fluorescence of Alexa Fluor-594. The pyramidal neuron is in an acute slice cut from of an Alpha-1E knock-out mouse. (B) Example of fluorescence collected during a line scan, shown in the yellow line in (A), which intersects the dendrite (den) and spine head (sp) during glutamate uncaging at the spine head. The increase in green signal indicates a rise in intracellular [Ca]. The inset traces show the simultaneously recorded uEPSP (top, amplitude 1.2 mV) and the quantification of the green fluorescence in the spine head (bottom, amplitude 10% ΔG/G_sat). (C) Average uEPSPs (top) and Ca-dependent changes in green fluorescence in the spine head (bottom) evoked by uncaging of glutamate in control conditions (black) and in the presence of SNX-482 (red). Data are shown as the mean (line) ± SEM (shaded region). (D) Summary of amplitudes of uEPSPs (left) and Δ[Ca]_spine (right) in control conditions (black) and in the presence of SNX-482 (red).