PMC

The colonies were established with new queens in Mississippi (MS) in April 2009, moved to South Dakota (SD) in May 2009, and finally to California (CA) in November 2009; monitoring concluded in January 2010.

(A) Arthropod pathogen microarray detection of viruses: sacbrood virus (SBV), black queen cell virus (BQCV), acute bee paralysis virus (ABPV), Israeli acute bee paralysis virus (IAPV), Kashmir bee virus (KBV), deformed virus (DWV) in each colony (5 bees per sample). (B) Incidence of select parasites assessed by end-point PCR from a single time-point each month (each chart n = 20, except January n = 17); the positive sample percentages in each pie-chart are indicated in red.

(A) Arthropod pathogen microarray detection of Nosema sp. in each colony (5 bees per sample) throughout the 10-month time-course. Colonies were managed using standard commercial beekeeping practices and treatments, which are listed below panel A and further described in . (B) Nosema ceranae and Nosema apis incidence assessed by species-specific end-point PCR from a single time-point (n = 20) each month; the positive sample percentages in each pie-chart are indicated in red. (C) Relative abundance of Nosema ceranae throughout the time-course assessed by qPCR of pooled monthly RNA samples; quantification of rRNA copy number based on a standard curve as described in .

(A) Light and fluorescent microscope images illustrate key features of this trypanosomatid parasite including DAPI stained kinetoplast DNA (yellow arrow) and nuclear DNA (white arrow), as well as the flagellar pocket (bottom panel, red arrow); scale bar = 5 µm. (B) Arthropod pathogen microarray detection of Crithidia mellificae in each colony (5 bees per sample) from October 2009 to January 2010. (C) Relative abundance of Crithidia mellificae throughout the time-course as assessed by RT-qPCR of pooled monthly time-course samples; quantification of rRNA copy number based on a standard curve as described in .

(A) RdRp amino acid phylogeny of the Nodavirales superfamily. Lake Sinai virus strain 1 (LSV1; HQ871931), Lake Sinai virus strain 2 (LSV2: HQ888865), chronic bee paralysis virus (CBPV; NC010711), boolarra virus (BoV; NC004142), Nodamura virus (NoV; NC002690), barfin flounder nodavirus BF93Hok (BFV; NC011063), grapevine Algerian latent virus (GALV; NC011535), melon necrotic spot virus (MNSV; NC001504), pothos latent virus (PoLV; NC000939) and carrot red leaf virus (CtRLV; NC006265). Protein sequences were aligned by ClustalW and a tree generated by the Neighbor-Joining method with 100 replicates (B) Genome organization of the Lake Sinai viruses and similar RNA viruses.

Viral genome copy numbers per 100 ng RNA were calculated based on standard curves [(black queen cell virus (BQCV), sacbrood virus (SBV), acute bee paralysis virus (ABPV), Lake Sinai virus strain 1 (LSV1), Lake Sinai virus strain 2 (LSV2), aphid lethal paralysis virus strain Brookings (ALP-Br), and Big Sioux River virus (BSRV)]; multiplying reported values by 500 provides a copy number per bee estimate, as further described in . LSV2, a novel virus, reached the highest copy number observed in this study in January 2010 (1.42×10⁹ copies per 100 ng of RNA sample; approximately 7.1×10¹¹ copies per bee); note the y-axes on each graph are independently scaled.