PMC

Rosettes of Col-0 (A) and amp1-1 (B) at 21 days after sowing. (C) Images of Col-0 and amp1-1 27 days after sowing. (D) Dry seeds from Col-0 and from amp1-1.

The structure of the AMP1 gene is presented. Exons are shown as boxes, T-DNA insertion locations are indicated by triangles and the amp1-1 and pt mutations by arrows.

ABA was extracted from seeds one week after harvest. Means of three biological replicates with their SE are shown. *Statistically significant difference in a Student's t-test (P<0.05; n = 4).

The percentage of genes observed and expected (considering random distribution) for each category is shown. The number of genes observed and expected is also indicated above each column. D (Dormant) and AR (After-Ripened) groups were defined in Cadman et al. (2006), containing 442 and 779 genes, respectively.

(A) % Germination seven days after sowing of bulk harvested Col-0 and amp1-1 (A), C24 and amp1-21 (B) and Ler and pt (C) after-ripened for the indicated time. (D) Germination of all amp1 mutant alleles in Col-0 background freshly-harvested seeds. Means of three biological replicates with their SE are shown. *Statistically significant difference in a Student's t-test (P<0.05; n = 3).

(A) AMP1 mRNA expression during seed development in Col-0. Under our experimental conditions seeds were considered mature at 14–15 days after pollination (B) Expression of several ABA-related genes and of AFL group of genes in Col-0, amp1-1, C24 and amp1-21 developing seeds at 10 days after pollination. Means of three biological replicates with their SE are shown. Letters above bars identify statistically significant different values for each gene in a Student's t-test (P<0.05; n = 3).