PMC

Analysis of B-cell maturation in cord blood of a normal newborn (control group). Expression of CD34 was used to identify immature B-cells. Maturation was studied using the expression of CD45, CD22, CD10 and membrane IgM (sIgM). A) CD45/CD34/CD19/CD22 combination - black: immature cells - CD45^lowCD34⁺CD19⁺CD22^- red: intermediate cells - CD45^-/+CD34^lowCD19⁺CD22⁺ yellow: mature cells - CD45⁺CD34^-CD19⁺CD22⁺. B) sIgM/CD34/CD19/CD10 combination - black: immature cells - sIgM^-CD34⁺CD19⁺CD10^-/+ red: intermediate cells - sIgM⁺CD34^-/+CD19⁺CD10^-/+ yellow: mature cells - sIgM⁺CD34^-CD19⁺CD10^-/+.

Analysis of T cell proliferation of non-stimulated (A) and PHA-stimulated (B) cord mononuclear cells of a newborn from a HIV-infected mother. After gating of CD3 cells, events were analyzed for size and complexity (forward-scatter and side-scatter gates), from which resting (R1-green) and blast cells (R2-blue) were separated. T-lymphocyte subsets were then analyzed. Dead cells (red) were excluded from all analyses. T cell proliferation was the difference between the percentages of PHA-stimulated blasts and non-stimulated blasts.