PMC

95% confidence intervals given for key TCA fluxes (ν_i) in the fumarate cultures (E3, E4), normalized by fumarate uptake. ν_net = ν₂ - ν₃.

Relative flux distributions (95% confidence interval) at key branch points of the central metabolic network for different experimental conditions of E1, E2, E3, and E4 (Table 1).

Comparisons of amino acid carbon mass isotopomer distributions between the experiments listed in Table 1. The straight line corresponds to a one-to-one (y = x) line. R: correlation coefficient; D: Euclidian distance.

In vivo flux estimates (95% confidence intervals given for the flux mean estimates) for the central metabolic network of G. sulfurreducens, corresponding to E1, E2, E3, and E4 (Table 1). All fluxes are normalized to acetate uptake (ACKr, PTAr). Gray double-headed arrows indicate bidirectional reactions, and the black arrowa with plus signs correspond to the net direction of a positive value for the bidirectional reactions. Unidirectional reactions were indicated by gray single-headed arrows. The subscript "ex" denotes extracellular metabolites. The abbreviations (pathway enzymes) are listed in Table 2, and precursor demand for biomass synthesis (precursors in yellow color) in Table 4.