PMC

ESCs reverse cardiomyopathy in WT/Mst1 chimeras. a–c Immunofluorescence showing detection of Mst1 in Mst1 transgenic (c) and WT/Mst1 chimeric (b) (note patchy areas of Mst1 staining in the WT/Mst1 chimera), but not in WT (a) heart sections. a inset: WB showing detection of Mst1 (red arrowhead) in Mst1 transgenic (right lane) and WT/Mst1 chimeric (center lane), but not in WT (left lane) hearts (blue arrowhead: tubulin control). d–f H&E staining showing reversion of dilated cardiomyopathy in the WT/Mst1 chimeric hearts (yellow dotted circle demarcates the left ventricle). g–i Masson Trichrome staining showing reduction of fibrosis (yellow arrows) in WT/Mst1 chimeric hearts. DAPI in blue (a–c) demarcates nuclei. Magnification: 100× (a–c); 10× (d–f) and 400× (g–i)