PMC

The root hair transcriptomes. B: genes preferentially expressed in trichoblasts compared to atrichoblasts; J: genes preferentially expressed in wild-type Arabidopsis roots compared to the root hair-defective rhd2 mutant; Bex: genes excluded from the analysis that yielded the B set because of irregular behavior during protoplasting.

The act11-1 mutation and its phenotype. (A) Map of the ACT11 locus, T-DNA location is marked by arrow. (B) Incomplete dominant fertility defect associated with act11-1. Average number of seeds per silique (± standard error of the mean) is shown for each genotype, with No. of siliques/No. of plants indicated below each bar. Values marked by the same symbol differ with t-test p < 10⁻⁴. The difference between hetero- and homozygous mutant plants was non-significant (p > 0.05). (C) act11-1 mutants have longer roots with denser root hairs than wild type and LatB increases root hair density in both genotypes. Average root length and root hair density (± standard error of the mean) is shown for each parent plant separately and for pool of seedlings of each genotype. Each bar represents data from at least ten plants (pooled data are from at least 28 plants). Pooled seedling values marked by the same symbol differ with t-test p < 0.005.

Composition of the analyzed data sets. Gene categories have been assigned manually based on gene annotations, GO keywords or homology to related proteins (see ). Categories likely to contain a considerable fraction of components of the AC D/RD module are shown in darker shades than those containing metabolic enzymes, components of miscellaneous regulatory or biosynthetic pathways and unknown or uncharacterized proteins. (A) The “doubly confirmed” (DC) and “all inclusive” (AI) root hair transcriptomes. (B) The virtual Arabidopsis root hair proteome. (C) A Venn diagram of the relationship between the AI transcriptome (AI), the virtual proteome (Proteome) and a set of 1,840 genes with highest expression levels in the root hair differentiation zone (Differentiation zone; DZ in ). (D) The set of genes shared by the AI transcriptome and the virtual proteome. (E) The set of genes shared by the “all inclusive” transcriptomes of root hairs/trichoblasts and pollen.