Cells were transfected with UCH-L1-HA for a total of 28 h. 5 µM MG132 was added for the times indicated prior to the end of the experiment.
A Typical example of cells overexpressing UCHL1-HA which contain inclusions. Images immunostained with monoclonal HA (green) and counterstained with DAPI to visualise nuclei (blue). Arrows indicate the position of inclusions within the cell.
B Time course for UCH-L1 inclusion formation. The presence of UCH-L1 was assessed by immunofluorescence using mouse monoclonal anti-HA antibodies. Bars represent the percentage of cells containing inclusions. Error bars indicate the standard error from the mean of the experiments. Asterisk indicates a significant difference between the percentage of untreated cells versus the percentage of treated cells. *p<0.001. no TX = no treatment.
C Comparison of experimental data and simulation results for the percentage of cells containing inclusions in each experiment. Error bars for simulated data represent the standard error for a percentage (s.e =
, where p is the percentage and n is the number of simulations).