PMC

Daytime (light areas) injection of BzATP enhanced duration of rat NREMS, EEG SWA during NREMS, and EEG spectral power. In contrast, duration of REMS was decreased during daylight hours but enhanced in the subsequent dark period. EEG power values were obtained during postinjection hours 1–6. ○, Values from control recordings; ●, values obtained after BzATP injections. *Significant differences between control and test days (P < 0.05, paired t-test).

Expression of P2X7 receptor mRNA in the somatosensory cortex and hypothalamus after 6 h of sleep deprivation (SD) or 2 h and 5 h after intracerebroventricular injection of 2.5 ng of IL1. Left: SD significantly decreased the levels of P2X7 receptor mRNA in the somatosensory cortex, and IL1 enhanced P2X7 mRNA levels both 2 and 5 h after the injection. Right: in the hypothalamus SD enhanced P2X7 mRNA levels, and IL1 significantly decreased P2X7 receptor mRNA. *Significant difference from corresponding control (C).

IL1 enhances duration of NREMS and inhibits EEG SWA during NREMS after intraperitoneal injections in P2X7 receptor KO and WT mice. ●, Data from IL1-treated mice; ○, values from saline-treated control mice. Unlike intracerebroventricular or intravenous injections in rats, intraperitoneal injections of IL1 reduced EEG power as previously reported (). The responses to IL1 were similar in both genotypes (WT, left; KO, right), suggesting that IL1's influence on sleep results from a step downstream from the ATP-P2 receptor interaction. *P < 0.05 difference between baseline and test days.

Sleep responses induced by sleep deprivation were attenuated in P2X7 receptor knockout (KO) mice compared with wild type (WT) control mice. Mice were deprived of sleep from 0600 to 1200 and then allowed to sleep ad libitum. WT mice exhibited typical NREMS rebound (right, inset) and EEG SWA enhancements (left) during the first 2 h after deprivation as previously reported (). In contrast, P2X7 receptor KO mice had attenuated NREMS and EEG SWA responses during the same period. Both strains had reduced EEG SWA for postdeprivation hours 6–22 (1800–1000) as previously reported (). *P < 0.05 difference between WT and KO mice for NREMS and EEG SWA. ○, Baseline values; ●, values after sleep deprivation.

Diurnal variation in the relative amount of interleukin-1β (IL1) mRNA and P2X7 receptor mRNA and protein in the hypothalamus and somatosensory cortex. A: Western blot analyses of somatosensory cortex samples showed variation of P2X7 receptor protein levels at 1500 (3 h after light onset) or 0300 (3 h after dark onset). No differences in time of day were observed in the standard, β-actin. B: time course of P2X7 receptor mRNA and protein (▴) and IL1 mRNA (●). The levels of P2X7 receptor protein (somatosensory cortex) and mRNA [(hypothalamus (□) and somatosensory cortex (▼)] were greater during the light period than during the dark period (black horizontal bar indicates dark period). Expression of protein levels was normalized to β-actin levels. *Statistical significance (P < 0.05) from values obtained at 2100; +significant differences from dark onset; #significant differences from values obtained 12 h before.

Both P2X7 receptor antagonists, injected at light onset (filled bars, ●), suppressed NREMS compared with saline injections (open bars, ○). oxidized ATP (OxATP) attenuated SWA during the first 12 h at the 1–4 Hz frequencies. Although EEG SWA was not significantly affected by A438079 during the first 6 h. With select 2-h time bins A438079 induced a biphasic effect, initially suppressing but then augmenting the EEG power spectra at different frequencies (see text for details, data not shown). Two smaller doses of A438079 were also tested, 1 nmol and 10 nmol; neither dose affected any of the parameters measured (*P < 0.05 between control and test days).

Extracellular ATP involvement in sleep regulation. ATP is released into the extracellular space as a consequence of cell activity during neuro- or gliotransmission. Extracellular ATP then activates P2 receptors (R), e.g., P2X7, that in turn are involved in IL1 processing and release as well as release of other sleep regulatory substances such as tumor necrosis factor and brain-derived neurotrophic factor (not shown). IL1, in turn, activates nuclear factor-κB (NF-κB), leading to changes in receptor trafficking. This changes the cell's long-term sensitivity to neurotransmitters such as glutamate (glu) and to neuromodulators such as adenosine. Extracellular ATP is also catabolized to adenosine via the actions of ectonucleotidases such as CD39 and CD73; this action is faster than the ATP-P2-induced changes in transcription and translation. Both actions of ATP are likely involved in sleep regulation. AMPA, amino-3-hydroxy-5-methyl-4-isoxazole proprionic acid.

Nighttime (shaded area) injection of 2′(3′)-O-(4-benzoylbenzoyl)adenosine 5′-triphosphate (BzATP) enhanced non-rapid eye movement sleep (NREMS). Rats received 1 of 3 doses of BzATP as indicated at dark onset. All 3 doses enhanced duration of NREMS during the initial 6–8 h after injection. The highest dose also enhanced duration of NREMS in the subsequent light period. BzATP inhibited duration of rapid eye movement sleep (REMS) during the daylight period occurring 12 h after the injection after the lower 2 doses. After the highest dose, REMS was enhanced during the first 4 h after injection. BzATP induced enhancement of electroencephalographic slow-wave activity (EEG SWA). BzATP at the lowest dose enhanced EEG delta power; this effect was absent after the higher doses. EEG power values were obtained during the first 6 h after injection. ○, Control values; ●, values obtained after BzATP injections. *Significant differences between control and test days (P < 0.05, paired t-test).