PMC

Nucleotide mismatch analysis of 42 Ovar-DRB1 alleles held in the sheep immunopolymorphism (IPD) data base www.ebi.ac.uk/ipd/mhc/ovar/index.html showing the degree of diversity between alleles represented in ESG1 and ESG2.

Alignment of sequences derived from four sheep DRA transcripts in comparison with the orthologous goat, cattle buffalo and pig sequences shown in Supplementary . Sequences are numbered from the first amino acid of the mature protein. Amino acid identity is represented by dots (.) and amino acids predicted to be associated with the peptide binding domain are highlighted with the + symbol. Ten sites under positive selection are labelled •. TM, transmembrane and CP, cytoplasmic domains.

4A Peripheral blood mononuclear cells derived from sheep homozygous for i; DRA*0201/DRB1*0901, ii; DRA*0102/0501 and iii; DRA*0101/0301 using DRα chain specific monoclonal antibody VPM 54. Horizontal axis FSC, forward scatter (cell size), vertical axis FL1, fluorescence channel 1. The percentage of positive events above the cut-off set based of the unlabelled population is shown in the top right. 4B Peripheral blood mononuclear cells derived from sheep homozygous for i; DRA*0201/DRB1*0901, ii; DRA*0102/0501 and iii; DRA*0101/0301 using DRβ chain specific monoclonal antibody VPM 57. 4C Peripheral blood mononuclear cells derived from sheep homozygous for i; DRA*0201/DRB1*0901, ii; DRA*0102/0501 and iii; DRA*0101/0301 using DQα chain specific monoclonal antibody VPM 36. 4D COS-7 cells transfected with haplotype matched and mismatched combinations of DRA/B genes. i; DRA*0201 and DRB1*0901, ii; DRA*0201 and 0501, iii; DRA*0201 and 0301 and iv; DRA*0201 only.

Species designations associated with the MHC nomenclature are as follows; Bibo, Bison bonasus (European Bison); Boga, Bos gaurus (Gaur); Boja, Bos javanicus (Banteng); Bubu, Bubalus bubalis (Asian Water Buffalo); Bude, Bubalus depressicornis (Lowland Anoa); Buta, Budorcas taxicolor (Takin); Cahi, Capra hircus, (Domestic Goat); Cafa, Capra falconeri (Markhor); Ceel, Cervus elaphus (Red Deer); Cota, Connochaetes taurinus (Blue Wildebeest); Deca, Delphinus capensis (Common Dolphin); Grgr, Grampus griseus (Risso's Dolphin); Orda, Oryx dammah (Scimitar Horned Oryx); Ovar, Ovis aries (Domestic Sheep); Ovca, Ovis canadensis, (Canadian Bighorn Sheep); Ovda, Ovis dalli (Dalli Sheep); Ovmo, Ovibos moschatus (Musk Ox); Phca, Physeter catodon (Sperm Whale); Ruru, Rupicapra rupicapra (Chamois); Rata, Rangifer tarandus (Reindeer); SLA, Swine Leucocyte Antigen (Pig); Syca, Synserus caffer (African Buffalo); Vipa, Vicugna pacos (Alpaca).