Kymographs (A, B and C) depicting mitochondrial motility were made from , , , , , , and , , respectively. In the experiment, a segment of axon was imaged continuously for 3 hours with a short break to allow for administration of drugs. Images were acquired at 10-second intervals. A. Treatment with tubacin. B. Treatment with TSA. C. Treatment with niltubacin. D, E and F. Quantification of the numbers of moving mitochondrial during the period of observation (n = 6). Only mitochondria that moved through the entire field of view were counted. G, H and I. Quantification of mean velocities of moving mitochondria (n = 5).