(A) Total B cells, as defined by CD19+ cells. The total B cell counts for TOL, SI, and HC cohorts were 287, 120, and 176 cells/μl, respectively. (B) Naive B cells, as defined by CD19+CD27–IgM+IgD+ cells. The naive B cell counts for TOL, SI, and HC cohorts were 190, 61, and 90 cells/μl, respectively. (C) Memory B cells, as defined by CD27+IgM+IgD+. The mean numbers for the memory B cell subset were 54.2 and 20.8 cells/μl for TOL and HC, respectively. (D) CD86+ B cells, as defined by CD86+CD19+. Mean numbers of CD86+CD19+ B cells for TOL and HC cohorts were 22 and 4.5 cells/μl, respectively. All values are shown as log2 absolute numbers, a calculation of the percent of lymphocyte gate multiplied by the total wbc count obtained from the same sample on a Coulter Counter. Boxes depict IQR; whiskers denote 1.5 × IQR; values beyond this range are shown as outliers. P values are shown for statistically significant differences.