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2.
Figure 4

Figure 4. Immunohistochemical expression of LRF in various breast diseases. From: Expression of Leukemia/Lymphoma-Related Factor (LRF/POKEMON) in Human Breast Carcinoma and Other Cancers.

DAB was used as a chromogen and counterstained with hematoxylin. Representative tissues from each disease are shown (200x – 400x).

Anshu Aggarwal, et al. Exp Mol Pathol. ;89(2):140-148.
3.
Figure 6

Figure 6. Immunohistochemical expression of LRF in various carcinomas and corresponding normal tissues. From: Expression of Leukemia/Lymphoma-Related Factor (LRF/POKEMON) in Human Breast Carcinoma and Other Cancers.

DAB was used as a chromogen and hematoxylin as a counterstain. Representative samples from the array are shown (200x – 400x).

Anshu Aggarwal, et al. Exp Mol Pathol. ;89(2):140-148.
4.
Figure 5

Figure 5. Immunofluorescence of LRF expression in breast diseases. From: Expression of Leukemia/Lymphoma-Related Factor (LRF/POKEMON) in Human Breast Carcinoma and Other Cancers.

Left Panels - Immunofluoresence using goat anti-rabbit Cy2 as the secondary antibody showing LRF expression, Right Panels - DAPI (blue) was used to a nuclear counterstain (200x – 400x).

Anshu Aggarwal, et al. Exp Mol Pathol. ;89(2):140-148.
5.
Figure 7

Figure 7. Immunofluorescence of LRF expression in various carcinomas and corresponding normal tissues. From: Expression of Leukemia/Lymphoma-Related Factor (LRF/POKEMON) in Human Breast Carcinoma and Other Cancers.

Left Panels - Immunofluoresence using goat anti-rabbit Cy2 as the secondary antibody showing LRF expression, Right Panels - DAPI (blue) was used to a nuclear counterstain. Representative samples from the array are shown (200x).

Anshu Aggarwal, et al. Exp Mol Pathol. ;89(2):140-148.
6.
Figure 3

Figure 3. LRF expression in malignant and normal adjacent tissues of a patient with breast cancer. From: Expression of Leukemia/Lymphoma-Related Factor (LRF/POKEMON) in Human Breast Carcinoma and Other Cancers.

A - Immunohistochemical expression of LRF. Sections were stained with DAB and counterstained using hematoxylin. B - Immunofluoresence of LRF expression using goat anti-rabbit Cy2 as the secondary antibody, C - DAPI (blue) was used to stain nuclei (400x).

Anshu Aggarwal, et al. Exp Mol Pathol. ;89(2):140-148.
7.
Figure 1

Figure 1. Expression of LRF and its mRNA transcripts in human benign and malignant breast biopsy tissues. From: Expression of Leukemia/Lymphoma-Related Factor (LRF/POKEMON) in Human Breast Carcinoma and Other Cancers.

A - Representative samples of LRF mRNA transcripts expression by RT-PCR with densitometric analysis. B - Representative samples of LRF protein expression by western blotting with densitometric analysis. C - Densitometric analysis of all twenty samples indicating significantly higher expression of LRF mRNA and protein in malignant tissues than in the benign tissues. (*p<0.05; **p<0.01)

Anshu Aggarwal, et al. Exp Mol Pathol. ;89(2):140-148.

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