PMC

Growth of V. mimicus. (A) Wild type V. mimicus CS-5 and (B) luxO disruptant mutant LODC 5. White staples indicate alone cultured bacteria and the grey co-cultured with A. castellanii. Data indicate mean values ± SD of three repeated experiments.

Growth of A. castellanii. White staples indicate growth of A. castellanii in the absence of bacteria, grey in presence of wild type V. mimicus CS-5, and black in the presence of luxO disruptant mutant LODC 5. Data indicate mean ± SD values of three repeated experiments.

Electron microscopy of the intracellular localization of V. mimicus CS-5 in A. castellanii. b, bacteria; n, nucleus; v, vacuole; x, ectocyst; y, mesocyct; z, endocyst. A. Acanthamoeba castellanii trophozoite in absence of bacteria. B. Vibrio mimicus CS5 localized in cytoplasm of A. castellanii trophozoite, 1 day after co-cultivation. C. Acanthamoeba castellanii cyst contains intracellular V. mimicus CS5, 1 day after co-cultivation. D. Acanthamoeba castellanii trophozoite contains intracellular V. mimicus CS5, 3 days after co-cultivation. E. Acanthamoeba castellanii cyst contains intracellular V. mimicus CS5, 3 days after co-cultivation. F. Acanthamoeba castellanii precyst contains intracellular V. mimicus CS5 in space between ecto- and mesocyst, 3 days after co-cultivation.