PMC

Yeast expressing EaDAcT accumulate acTAGs but not lcTAGs. Positive-ion ESI mass spectra of neutral lipid extracts from yeast strains InvSc1 (A) or H1266 (B) expressing the empty vector pYES-DEST52, EaDAcT, or EaDGAT1. Tritridecanoin (tri13:0) and triheptadecanoin (tri17:0) were added as internal standards.

EaDAcT possesses acetyltransferase but not oleoyltransferase activity in vitro. (A) TLC separation of lipid extracts from yeast microsomes (InvSc1 background) expressing EaDAcT, EaDGAT1, or empty vector (pYES-DEST52) incubated with 15 μM [¹⁴C]acetyl-CoA in the presence or absence of exogenous 230 μM dihexanoin. (B) TLC separation of lipid extracts from yeast microsomes (H1266 background) expressing EaDAcT, EaDGAT1, or empty vector (pYES-DEST52) incubated with 8 μM [¹⁴C]oleoyl-CoA.

Arabidopsis seeds expressing EaDAcT accumulate acTAGs. (A) Positive-ion ESI mass spectra of neutral lipid extracts from Col-0 wild-type seed or T₃ seed from a representative Col-0 plant expressing EaDAcT. Tritridecanoin (tri13:0) and tripentadecanoin (tri15:0) were added as internal standards. (B) Scatter plot showing the distribution of the acTAG composition of T₃ seed from different transgenic Col-0 plant lines expressing EaDAcT or of Col-0 wild-type seed. Horizontal lines represent the median value for each sample group. (C) ESI-MS² analysis of neutral lipid extracts from the T₃ seed of Col-0 plants expressing EaDAcT. Shown are the daughter fragment peaks from acTAGS with [M + NH₄]⁺ adducts with m/z values of 653 and 677.

Euonymus alatus fruit synthesize different types of TAGs in a tissue specific manner. (A) Cross-section of a developing fruit from Euonymus alatus illustrating the different TAG producing tissues. a, aril; em, embryo; en, endosperm. Bar represents 1 mm. (B) Positive-ion ESI mass spectra of lipid extracts from Euonymus alatus aril and seed tissues, demonstrating the accumulation of lcTAGs (highlighted in orange) in the aril and acTAGs (highlighted in green) in the seed. Peaks correspond to m/z values of the [M + NH₄]⁺ adduct. Tripentadecanoin (tri15:0) was added as an internal standard. The number of acyl carbons in each series of TAG molecules is indicated; the number of double bonds (x) is not defined.