PMC

The log expression levels (low to high) are plotted on the Y-axis. The X axis reflects an arbitrary tumor number, grouping the different tumor types starting with the congenital mesoblastic nephromas (infantile fibrosarcomas) in black, followed by clear cell sarcoma of the kidney in green, rhabdoid tumors in red, and Wilms tumors in blue.

A. Immunoblotting of CDH2, NCAM, LGALS1, MYC-C and TFRC
Protein lysates from 3 examples of each tumor type were analyzed using antibodies provided in . Decreased expression of neural markers CDH2 and NCAM is identified in RT. Increased expression of oncogene LGALS1 is identified in 2/3 RT tested, as well as in two CMNs. Increased expression of MYC-C and one of its key targets (TFRC) is identified in RT.
B. Nestin expression in RT.
Nestin mRNA was not differentially expressed in the pediatric renal tumors; however Nestin protein cytoplasmic and nuclear expression was identified in all RT tested using immunohistochemistry.

RNA from 7 examples of each tumor type were analyzed in duplicate using the primers in . Expression changes are determined by subtracting the Ct value of the housekeeping gene (B-actin or GAPDH) from the Ct of the gene of interest (dCt). The average dCT from each individual tumor was subtracted from the average tumor RT dCt value for each gene (ddCT); these values were averaged for each tumor type and the error bars represent the standard deviation of the averages.

A) Pattern of RNA expression of key genes. The expression levels (low to high) are plotted on the Y-axis. The X axis groups the different tumor types starting with the congenital mesoblastic nephromas (infantile fibrosarcomas) in black, followed by clear cell sarcoma of the kidney in green, rhabdoid tumors in red, and Wilms tumors in blue.
B) Protein expression of SPP1 and PEDF (SERPINF1). The majority of cells within all RT show moderate positivity for SPP1. Note the striking positivity of non-neoplastic renal tubules for SPP1. Clusters of cells within RT show strong positivity for PEDF.