PMC

Adsorption of pMcm2 antibody confirms specificity to corresponding pMcm2 antigen. (A) AD hippocampal tissue stained with pMcm2 antibody. (B) Adjacent section treated with pMcm2 antibody absorbed with non-phosphorylated Mcm peptide demonstrates similar staining. (C) Adjacent section treated with pMcm2 antibody absorbed with phosphorylated Mcm2 peptide demonstrates complete absorption. * denotes landmark vessel.

Pretreatment with alkaline phosphatase to remove phosphate groups, results in elimination of pMcm2 reactivity (B) compared to an untreated adjacent serial section of an AD case (A). * denotes landmark vessel. Scale bar = 50 μm.

In another AD case, age 63, adjacent hippocampal tissue sections demonstrate many of the AD-related pathological structures (arrows) containing pMcm2 (A) are also positive for hyper-phosphorylated tau (B) in the CA1 region. Lower magnification of adjacent sections of the subiculum shows the large number of NFT and plaques recognized by pMcm2 (C) and AT8 (D). * denotes landmark vessel. Scale bars= 50 μm (A,B), 100 μm (C,D).

In an 87 year old AD case, hippocampal tissue sections demonstrate significant localization of pMcm2 protein in NFT, dystrophic neurites, and neuropil threads (B). In another AD case, in the CA3 region, in addition to pathological structures, a few pyramidal neuron nuclei (arrows) have significant pMcm2 accumulation (D). Most control cases, representative case age 61 years, demonstrate no neuronal staining for pMcm2 protein (A), while a few older control cases demonstrate significant nuclear immunolocalization in the pyramidal neurons (control case age 74 years, C) Scale bar= 50 μm.