Illustration of AAV vector genomes. The stuffer fragments were partial sequences of factor VIII gene. The vectors were named according to their rAAV genome size. The DNA fragment used for the probe for Southern blot hybridization was identified, which corresponds to the sequences in the ampicillin-resistant gene. AAV, adeno-associated virus; ampR, an ampicillin-resistant gene, β-lactamase; ITR, inverted terminal repeat sequence; ori, a plasmid replication region of pUC18; ↓, AleI site; *, BamHI site; Δ, SmaI site.