The age-1(mg44)-F2 transcript profile differs from those of F1 adults or N2 dauer larvae. We retested 13 age-1-regulated genes, in an expanded strain panel. Strains are as described in the legend to , with the addition of age-1(mg44) F1 (fertile first-generation homozygotes), daf-2(e1370), a long-lived mutant of the insulin/IGF-1 receptor (G–M), and daf-16(mu86); age-1(mg44), in which a daf-16 deletion allele, mu86, replaces the m26 allele used in and . Double mutants with daf-16(mu86) were assessed only for those genes that had appeared to be incompletely (or negligibly) reverted by daf-16(m26) (F, H–M; indicated by black striping on green bars). Orange bars [at the right of (G–M)] represent N2DRM dauer larvae, induced by crowding on starved plates and confirmed by > 95% resistance to 1% sodium dodecyl sulfate for samples of 500 worms. Sample numbers (n = the number of independent biological preparations) were as follows: 8–11 groups of postgravid N2DRM adults, 4–5 each of age-1(hx546) and daf-16(m26); age-1(hx546), 3 of age-1(mg44) F1, 10–14 of age-1(mg44)-F2 adults, 5–6 each of daf-16(m26); age-1(mg44) and daf-16(mu86); age-1(mg44), 3–4 of N2DRM dauers, and 3–4 of daf-2(e1370).