Expression of Dicer in metastasis progression cellular model and in bone metastatic cells.
Expression of Dicer in the mouse metastasis progression cellular model: (
A) western blot analysis of Dicer expression in the five mouse mammary cell lines, namely 67NR, 168FARN, 4TO7, 66c14, and 4T1. The signal intensity of Dicer was normalised to that of actin. (
B) The relative levels of Dicer mRNA were measured by real-time RT–PCR, and each bar represents the mean±s.d. of the PCRs in triplicate (▪). Dicer protein levels were quantified using Quantity One software (BioRad, Marnes-la-Coquette, France) and expressed as protein relative quantity. The ratios of Dicer/actin of three independent studies were expressed as mean±s.d. (
). E, epithelial phenotype; M mesenchymal phenotype; E/M, mixed phenotype.
Expression of Dicer in two bone metastatic derivatives of MDA-MB-231 cells: (
C) western blot analysis of Dicer expression in the three cell lines, namely MDA-MB-231, BO2, and SCP2. The signal intensity of Dicer was normalised to that of actin. (
D) The relative levels of Dicer mRNA were measured by real-time RT–PCR, and each bar represents the mean±s.d. of the PCRs in triplicate (▪). Dicer protein levels were quantified using Quantity One software (BioRad, Marnes-la-Coquette, France) and expressed as protein relative quantity. The ratios of Dicer/actin of three independent studies were expressed as mean±s.d. (
).