PMC

Graphs showing stimulation index results and interferon-γ production of human immunodeficiency virus-1⁺ patients' peripheral blood mononuclear cell stimulation with peptide pools of Gag 20mers and Gag 9mers. The first set of graphs show results using anti-programmed death (PD-1) antibody in lymphocyte proliferative response (LPR) and enzyme-linked immunospot assays (ELISPOT) (a); the last four graphs show LPR and ELISPOT assays using anti-PD-ligand-1 (b).

Representation of data for flow cytometric analysis of expression of programmed death ligand (PD-L1) molecule on CD4⁺[a: percentages; b: mean fluorescence intensity (MFI)] and CD8⁺ T cells (c: percentages; d: MFI). Bar graphs show mean and error bars represent standard error of the mean in the three different groups: healthy controls (▭), aviraemic patients () and viraemic patients (). The histograms show PD-L1 expression on the T cell subsets from a representative human immunodeficiency virus-1⁺ aviraemic patient (f).

Cumulative data for flow cytometric analysis of expression of programmed death (PD)-1 molecule on the different memory subsets on CD4⁺[a: percentages; b: mean fluorescence intensity (MFI)] and CD8⁺ T cells (c: percentages; d: MFI). Bar graphs show the mean, and error bars show standard error of the mean, in the three different groups: healthy controls (▭), aviraemic patients () and viraemic patients (). Histograms show PD-1 expression on CD4 and CD8 T cells from a representative human immunodeficiency virus-1⁺ aviraemic patient (f) in the different T memory subsets.

The plots show gating strategy for T cell memory subsets on CD4 and CD8 T cells of a representative human immunodeficiency virus (HIV)-1⁺ aviraemic patient (a). The graphs show combined data for flow cytometric analysis of distribution of naive, central memory T cells (T_CM), effector memory T cells (T_EM) and terminally differentiated effector T cells (T_EMRA) subsets in CD4⁺ (b) and CD8⁺ (c) T cells in healthy donors (▭) compared to HIV-1⁺ aviraemic patients () and HIV-1⁺ viraemic patients (). Box plots on graphs show median (central bar), interquartile range (IQR) (box), 10th and 90th percentile (whiskers) and outliers (black dots).