PMC

Immunolocalization of ERα in seminoma (A), embryonal carcinoma(B) and control testis (C); the pictures show representative samples. L, linfocytes. Lc, Leydig cells. Nc, neoplastic cell. T, seminiferous tubules. Scale bars = 12,5 mμ.

PLAP staining and immunolocalization of estrogen receptors (ERβ1, ERβ2) in intratubular germ cell neoplasia adjacent to seminoma (A) and embryonal carcinoma(B); the pictures show representative samples. A, B: PLAP staining. A1, B1: ERβ1 immunolocalization. A2, B2: ERβ2 immunodetection. Ncg, neoplastic germ cell. Sc, Sertoli cell. Scale bars = 5 mμ.

Immunoblots of estrogen receptors (ERα, ERβ1, ERβ2) from representative protein extracts of seminoma and embryonal carcinoma. ERα immunoblot (A): MCF7 cultured cells (C), seminoma (lane 1), embryonal carcinoma (lane 2) and control testis (lane 3). ERβ1- ERβ2 immunoblot (B): LnCap cultured cells (C), seminoma (lane 1), embryonal carcinoma (lane2) and control testis (lane 3). β-actin serves as a loading control. Numbers on the left-hand side correspond to the standard molecular weights Numbers on the right-hand side correspond to molecular weights of the detected protein.

Morphology and immunolocalization of estrogen receptors (ERβ1, ERβ2) in seminoma, embryonal carcinoma and control testis. The pictures show representative samples. A, A1, A2: Haematoxylin-Eosin staining. B, B1, B2: ERβ1 immunolocalization. C, C1, C2: ERβ2 immunodetection. Scale bars = 12,5 m μ. L, linfocytes. Lc, Leydig cell. Nc, neoplastic cell. Pc, plurinuclear cell. S, septa. T, seminiferous tubule. Inserts at the bottom right side (B, B1, B2, C, C1, C2): absorption controls. Scale bars = 12,5 mμ. Inserts at the top right side (B, B1, C, C1): higher magnification of the pictures. Scale bars = 5 mμ.