PMC

(A) Collagen-induced platelet aggregation from mice maintained on a high fat diet for 6 months. (B) Summary of 16 studies performed with platelets from 5 mice of each genotype (mean ± SEM).

(A) Whole blood anticoagulated with PPACK was perfused through the chamber at 1000 s^-1 for 300 seconds, then platelet poor plasma was substituted and shear was increased 10-fold. Mean ± SEM (N=7). (B) Data at 10,000 s^-1 were normalized by dividing by the fluorescence intensity achieved just before the shear increase.

(A) Aortas after 6 months on the high fat diet showing lipid-rich deposits. (B,C,D) Analysis of lesion size. (E) Sections from the aortic root. Markers: CD4 and CD8, T-cells; CD22, B-cells; CD11b, monocytes, macrophage and neutrophils; smooth muscle actin (αSMA), smooth muscle cells and fibrotic caps. No differences were observed.

Platelet accumulation (green) in cremaster muscle arterioles damaged with a pulsed laser. (A&B) Video captures 2 min after injury. The WT and sema4D(-/-) mice were on a normal diet. LDLR(-/-) and sema4D(-/-)LDLR(-/-) mice were studied after 6 months on a high fat diet. (C&D) Median fluorescence intensity. WT: 29 injuries in 4 mice. Sema4D(-/-): 23 injuries in 3 mice. LDLR(-/-): 26 injuries in 3 mice. Sema4D(-/-)LDLR(-/-): 25 injuries in 3 mice. (E) Mean thrombus area. (F) Frequency of complete occlusion.