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1.
Fig. 4

Fig. 4. From: Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei.

Graphic depiction of the (a) EVD and (b) aspect ratio results from T-matrix fitting, Mie theory fitting, and QIA.

Cyrus Amoozegar, et al. Appl Opt. ;48(10):D20-D25.

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2.
Fig. 1

Fig. 1. From: Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei.

Bright-field image of a single 12 μm bead after stretching in (a) TM orientation and (b) TE orientation and diagram indicating the scattering geometries.

Cyrus Amoozegar, et al. Appl Opt. ;48(10):D20-D25.

CitationFull text

3.
Fig. 2

Fig. 2. From: Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei.

Schematic of the a/LCI system (taken from [], with permission). Serial scanning of the retroreflector (RR) and L4 enables depth-resolved mapping of the angular light scattering distribution from the sample.

Cyrus Amoozegar, et al. Appl Opt. ;48(10):D20-D25.

CitationFull text

4.
Fig. 3

Fig. 3. From: Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei.

(Color online) a/LCI data processing method. The optical depth corresponding to the spheroidal scatterers is integrated to obtain scattered light intensity as a function of scattering angle. The distribution is then low-pass filtered and a second-order polynomial is subtracted to detrend the data. The processed signal is compared to a database of scattering distributions calculated by Mie theory or the T-matrix method in order to determine the properties of the scatterers. In this case, the T matrix is used, and the fitting algorithm yielded an EVD measurement of 12:3 μm with an aspect ratio of 0.86.

Cyrus Amoozegar, et al. Appl Opt. ;48(10):D20-D25.

CitationFull text

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