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1.
Figure 1

Figure 1. From: Human MLPA Probe Design (H-MAPD): a probe design tool for both electrophoresis-based and bead-coupled human multiplex ligation-dependent probe amplification assays.

Outline of probe selection workflow. A successful probe set needs to meet all the criteria specified in the diagram.

Jizu Zhi, et al. BMC Genomics. 2008;9:407-407.
2.
Figure 2

Figure 2. From: Human MLPA Probe Design (H-MAPD): a probe design tool for both electrophoresis-based and bead-coupled human multiplex ligation-dependent probe amplification assays.

Score calculation for probe sets. The final score for each probe set is determined by the scores of Tm, ΔG, GC content and ligation site. Since each individual score falls in the range [0, 1], the final score should also fall in range [0, 1], with 1 being the best score. Probe sets with a final score > 0 are processed for further tests.

Jizu Zhi, et al. BMC Genomics. 2008;9:407-407.

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