PMC

PBLs from HIV infected individuals were isolated and treated with skTRAIL or BSA in a limiting dilution micro co-culture. HIV viral burden is reported as infectious units per million.

Peripheral blood lymphocytes (PBLs) were collected from HIV-negative donors, stimulated with PHA and IL-2, and treated with with skTRAIL at the indicated concentrations. Viability was assessed by trypan blue staining.

PBLs were collected from HIV-negative donors and infected with HIV-IIIB prior to treatment with skTRAIL. The effects on cell count (A), proliferation as assessed by CFSE dilution (B), p24 production (C), and CD69 expression (D) were measured.

PBLs from HIV infected subjects were cultured with skTRAIL or BSA and analyzed for percent of T, B and NK cells (A, top panel), and percent CD4+ and CD8+ memory and naïve T cells (A, bottom panel) by flow cytometry. Cultured PBLs were also analyzed for CD4 and CD8 recall responses by ELISPOT (B).

PBLs were collected from 4 HIV-infected donors and 1 uninfected control. Cells received either no treatment or were treated with either BSA (5 ng/ml) or skTRAIL (5 ng/ml). Following 24 h of culture, supernatants were collected for measurement of cytokine expression. Values are presented as relative fluorescence units, reflecting a range of concentrations from 0.08 to 2.01 pg/ml based on extrapolation from a standard curve generated with known concentrations of IL-10 at the time of analysis.