The anti-Htt intrabodies reduce mHDx-1-induced toxicity and aggregation in cell culture. A, The epitopes in HDx-1 for the various intrabodies are depicted. B, To quantify mHtt toxicity, HEK293 cells were cotransfected with mHDx-1-GFP and intrabody at various intrabody/Htt ratios and incubated for 48 h. Cells were stained with EthD-2 to identify dead cell nuclei, fixed, and stained with DAPI to identify all cell nuclei. The number of dead cells was normalized to total cell number. All of the intrabodies reduce mHDx-1-induced cell death in a saturable, dose-dependent manner, with maximal effects at different intrabody/Htt ratios (1:1 for VL12.3, 2:1 for Happ1 and Happ3, and 4:1 for MW7). C, Aggregation was determined by counting GFP foci and normalizing to total cell number. *Different from VL12.3 at p < 0.05; **p < 0.01. The point labeled as 0 on the intrabody:Htt axis corresponds to the value for HDx-1 + CVL.