PCR products were about 70 bp in GV, MI, MII oocytes, day2 (D2), day 3(D3), day 5(D5) embryos and spermatozoa (S). M: marker. NLRP1 was detected in GV, MI, MII oocytes and D2 embryos, but not in later stages. NLRP2, 4, 7, 11, 12 and 14 were detected in all samples. NLRP3 was only detected in GV, MI oocytes and spermatozoa. NLRP5, 8, 9, 13 were detected in all oocytes and embryos, but not in spermatozoa. NLPR6 was only detected in spermatozoa and NLRP10 was not detected in any gametes or embryos (figure not shown).