Expression data for ERG exons 2 to 11 in prostate cancers determined using GeneChip Human Exon 1.0 ST arrays. Cancers scored as having altered ERG expression profiles based on the statistical analyses presented in are marked with an asterisk. In cancers designated as having altered ERG expression profiles, exon 8 of ERG was always expressed at lower levels than exons 4 to 7 and exons 9 to 11, indicating that this exon can be spliced out in rearranged ERG transcripts. In many of the cancers without altered ERG expression profiles, exon 7 was expressed at lower levels than exons 2 to 6 and exons 8 to 11, consistent with the presence of splice variants containing deletion of this 81-bp exon. The apparently depressed level of expression observed in some cancers (eg, PRC9) reflects the variation in observed level of ERG expression across the 27 cancers observed in this study, as illustrated in , and was not believed to reflect an abnormal ERG expression pattern. For each probe set within an exon, the log2 ratio of the expression observed in the cancer to that observed in control non-neoplastic prostate epithelium was calculated. For each exon, the black dot represents the median of these values.