PMC

Serum was obtained from infected Wt or TIRAP-/- mice, 3 months after intravenous infection with 5×10⁵ BRD509, and from naïve Wt mice. IgG2a responses to Salmonella were determined by ELISA. Data show mean Salmonella-specific IgG2a titers ± SD of 8-9 mice per group.

C57BL/6 Wt and TIRAP-/- mice were infected intravenously with 5×10⁵ attenuated Salmonella, BRD509. (A) Spleens from infected mice were recovered at regular intervals after infection and the number of bacteria determined by plating serial dilutions. Data show the number of bacteria per spleen and are representative of at least 3 mice per group. (B) Spleens from infected mice were recovered 35 and 49 days after infection and the number of bacteria determined by plating serial dilutions. Data show the number of bacteria per spleen and are representative of at least 3 mice per group.

C57BL/6 Wt and TIRAP-/- mice were infected intravenously with 5×10⁵ attenuated Salmonella, BRD509 and 50 days later naïve or vaccinated mice were challenged intravenously with 1×10⁵ virulent Salmonella SL1344. Cages were monitored daily for death or moribund mice. Data show percentage survival of mice using combined data from 2 individual experiments and at least 10 mice per group.

Wt and TIRAP-/- mice were adoptively transferred with SM1 T cells and infected intravenously the following day with 5×10⁵ attenuated Salmonella, BRD509 or immunized intravenously with 1×10⁸ HKST. Spleens were harvested at various time points later and stained using antibodies specific for CD4 and CD90.1. FACS plots show the percentage of SM1 T cells in the spleen of uninfected (Transfer Only), HKST immunized (HKST), and infected (Salmonella) mice. Numbers are the percentage of SM1 cells among all live gated spleen cells. Data are representative of 3 mice per group and 2 individual experiments.

C57BL/6 Wt and TIRAP-/- mice were infected intravenously with 5×10⁵ attenuated Salmonella, BRD509. (A) Spleens from infected mice were harvested 35 days later and cultured in vitro in medium alone (-Ag) or with added HKST (+Ag). Supernatants were harvested 72 hours later and IFN-γ production measured by ELISA. Data shows the mean OD ± SD for 3 mice per group. (B) Spleens from infected mice were recovered 35 days after infection and stained with antibodies specific for cell-specific surface markers. Data show FACS plots for individual mice and are representative of 3 mice per group. Numbers show the percentage of cells within each boxed gate.

Wt and TIRAP-/- mice were adoptively transferred with CFSE-dyed SM1 T cells and infected intravenously the following day with 5×10⁵ attenuated Salmonella, BRD509 or immunized intravenously with 1×10⁸ HKST. Spleens were harvested at various time points later and stained using antibodies specific for CD4 and CD90.1 to identify SM1 cells, and CD11a to identify activated T cells. FACS plots show CFSE-dye dilution and CD11a expression on gated SM1 T cells in the spleen of uninfected (Transfer Only), HKST immunized (HKST), and infected (Salmonella) mice. There were too few SM1 cells at day 18 in infected mice to gather sufficient data for analysis. Data are representative of 3 mice per group and 2 individual experiments.