PMC

Phylogenetic tree. The phylogenetic tree of sequence R representing the four B19 virus isolates R1 to R4 was visualized by TreeView following alignment in BioEdit and Clustal W as shown in Figure 2. See legend to Figure 2 for accession numbers.

Agarose gel electrophoresis pattern of parvovirus B19 DNA amplicons. (A) 398 bp DNA bands obtained in the first round of PCR; (B) 288 bp DNA bands obtained in the second round of PCR. M: molecular weight marker (100 bp ladder, Invitrogen). P: positive control; lanes 1 to 4: amplicons of sera from patients 1 to 4, respectively.

Alignment of the partial DNA sequence of the four B19 isolates with reference strains. The partial VP1/VP2 DNA sequence (R) representing the four B19 virus isolates R1 to R4 was aligned with representative sequences of the three human parvovirus genotypes retrieved from GenBank. Accession numbers were as follows. Genotype 1: OsFr [GenBank: DQ225150.1]; Kati 4 [GenBank: AF161226.1]; AnTo [GenBank: DQ225151.1]; SN807 [GenBank: DQ225149.1]; NAN [GenBank: AY504945.1]; J35 [GenBank: AY386330.1]; B19-Au [GenBank: M13178.1]. Genotype 2: LaLi [GenBank: AY044266.1]; Berlin [GenBank: AJ717293.1]; BN31.2 [GenBank: DQ333426.1]; IM-81 [GenBank: AY903437.1]; A6c8 [GenBank: AY064476.1]. Genotype 3: V9 [GenBank: AY345134.1]; D91.1 [GenBank: AY083234.1]; BN30.3 [GenBank: DQ408305.1]; R0748 [GenBank: DQ234779.1].