PMC

Expression level of MSRA gene in the tumor tissues and cell lines of human hepatocellular carcinoma. A and B: MSRA mRNA levels of clinical specimens (T1-20 were HCCs without metastases, M1-20 were HCCs with metastases) (A) and HCC cell lines (B) detected by real-time PCR. C: MSRA protein levels detected by Western blotting (T1 to T3, T18, and T19 were HCCs without metastasis, M14 to M18 were HCCs with metastasis).

In vitro effect of MSRA overexpression on the proliferation and invasion of HCC cell line. RT-PCR (A, upper) and Western blotting (A, low) showed that the expression level (both RNAs and proteins) was significantly higher in HCCLM6 transfected with pIRES2-EGFP-MSRA (M) compared with the control that transfected with pIRES2-EGFP (P) and HCCLM6 cell line (LM6). There was no significant difference in proliferation of HCC cells between the MSRA-transfected group and the controls (B), however, the colon formation in the recombinant vector-transfected cells were less than in the control (C). In in vitro matrigel invasion assay, the invasion ability of HCC cells was significantly decreased in HCCLM6 cells transfected with the MSRA gene (D), compared with those the transfected with empty vector (E) and the untransfected HCCLM6 cells (F).