PMC

WT and STAT1^−/− mice were infected with 10⁸ spores B.anthracis intratracheally and monitored for survival. p = 0.13. 8–10 mice/group

Mice were administered B.anthracis 10⁸ spores/mouse and plasma harvested at 24 hrs. IL-6/10/12p40 determined by ELISA. N = 5/group.

C57BL/6 mice were given 10⁸ spores 34F₂ intratracheally in 100 µl saline. IFN-γ or IFN-α at described doses was added to saline and administered with B.anthracis spores. Mice were subsequently monitored for survival. N = 5–7 mice/group.

Mice were administered B.anthracis 10⁸ spores/mouse+IFN-γ (1 µg/ml), IFN-α (10⁴U/ml) or vehicle and BALF cells harvested at 24 hrs. For immunoblot. A. Immunoblot for MKK3. B. Immunoblot for phosphor-Tyr⁷⁰¹ and Total STAT1. Data represent pooled BAL from 5 mice. All lanes were normalized for protein (50 µg/lane)

Mice were administered B.anthracis 10⁸ spores/mouse+IFN-γ (1 µg/ml), IFN-α (10⁴U/ml) or vehicle and BALF and plasma harvested at 24 hrs. IL-6/10/12p40 determined by ELISA. N = 5/group. *p<0.05 compared to WT.- = levels in uninfected mice

Mice were infected with B.anthracis (10⁸)±IFN. A. Lungs were harvested at 24 hrs and the fraction of germinated spores determined by the formula (CFU B.anthracis in lung-CFU B.anthracis lung after heat treatment{dormant spores})/CFU B.anthracis lung. B. Quantitative culture from spleen harvested at 24 hrs. Serial dilutions were made of whole splenic homogenate. * p<0.05 compared to WT. N = 5/group.

Mice were infected with B.anthracis (10⁸) and A. Lungs were harvested at 24 hrs and the fraction of germinated spores determined by the formula (CFU B.anthracis in lung-CFU B.anthracis lung after heat treatment{dormant spores})/CFU B.anthracis lung. B. Quantitative culture from spleen harvested at 24 hrs. Serial dilutions were made of whole splenic homogenate. * p<0.05 compared to WT. N = 5/group