Representative patterns of isolates that had a mutation(s) according to the LiPA for detection of pncA mutations. Positions of the oligonucleotides, conjugate control lines, and marker lines are shown. A negative signal is shown by an open triangle. LiPA patterns of M. tuberculosis strains are shown in lanes 1 to 21. Lanes: 1, H37Rv; 2, IMCJ(130); 3, IMCJ904III; 4, IMCJ(66); 5, IMCJ(85); 6, IMCJ(67); 7, 13229; 8, IMCJ.M22; 9, P26; 10, 13243; 11, IMCJ850; 12, IMCJ835; 13, IMCJ501; 14, IMCJ844; 15, IMCJ479; 16, IMCJ843; 17, P12; 18, IMCJ901III; 19, IMCJ(80); 20, P10; 21, IMCJ.K1; 22, M. bovis BCG Japanese strain 172; 23, no DNA. WT, wild-type pncA.