PMC

(a) Representative recordings in Xenopus oocytes for the establishment of dose–response curves. (b) Dose–response curves constructed from mean values of three to five experiments fitted by the Hill equation.

Representative current traces illustrating the screening of monoterpenes for TRPV3 activation. HEK293 cells transfected with TRPV3 were held in the whole-cell voltage-clamp mode at −40 mV and superfused as indicated. (a) Representative response of one cell to dihydrocarveol and camphor. (b) Representative response of the same cell to (+)-dihydrocarvone. (c) Assay in an untransfected cell. Note that neither camphor nor the other two monoterpenes evoked any current. (d) TRPV3 expressing cell superfused with icilin.

Activation of TRPM8 by potent TRPV3 monoterpenoid agonists. (a) Representative current trace of a TRPM8 expressing HEK293 cell. The cell was held in the voltage clamp mode at −40 mV and transiently superfused with (−)-menthol (2 mM) and carveol (2 mM) as indicated. (b) Equivalent stimulation of an untransfected cell did not result in any response to either (−)-menthol or carveol (c) Summary of the evoked currents relative to that evoked by (−)-menthol; all compounds were applied at a concentration of 2 mM. Values shown are means±s.d.