PMC

Analysis of 34 mouse transcripts targeted by 2 distinct RNG/MRC probes. Shown are probes with a variation in intensity greater than 2 fold. Each number corresponds to the number of transcripts for which fluorescence intensity varied along with EST_NUMBER, DIST_TO_3′ and/or Tm.

Distribution of the probes according to their DIST_TO_3′ score. (A) human. (B) mouse. More than 90% of probes for the human sets and 98% of probes for the mouse sets are located within 1,500 bases from the 3′-end of target mRNAs. Legend indicates the average DIST_TO_3′ score for each collection. This comparison has been performed on a subset of 16,303 human and 13,073 mouse transcripts, common to all sets.

Blast-specificities of the different probe collections. (A) Average X_HYBRID scores for the different human and mouse collections. (B) Percentage of probes in each set associated with a X_HYBRID above 2, i.e. less ‘BLAST-specific’. This comparison has been performed on a subset of 16,303 human and 13,073 mouse transcripts, common to all platforms. ALL represents the collection of all probes calculated with OligoArray2.0. RNG/MRC represents the selection of probes used for the fabrication of the microarrays.

Matches with human and mouse EST databases for the different probe collections. (A) Average EST_NUMBER scores for the different human and mouse collections. (B) Percentage of probes matching no ESTs for All MEDIANTE probes, for the RNG/MRC, Agilent, Illumina and Affymetrix probe sets. For human, the comparison was performed on a subset of 7,325 transcripts having ‘BLAST-specific’ probes in all sets, i.e. X_HYBRID lower than 2.0. For mouse, the comparison was performed on a subset of 6,358 such transcripts. A matching EST was defined by a 95% identity between one probe and an EST.

Scatter plot of the ratios measured on Affymetrix GeneChip™ (x-axis) and on RNG/MRC microarrays (y-axis). RNA was derived from either HEK293 cells or a keratinocyte cell line (DK7). 11053 transcripts had at least one Affymetrix probe set and one RNG/MRC probe. Among them, 7054 pairs were further analyzed, as their intensity level was larger than the 25^th percentile on both platforms. After quantification of the signals on both platforms, the ratio of the expression levels between the two cell lines was established. The coefficient of correlation was equal to 0.81.

MEDIANTE screenshot of the summary data for transcript NM_001652. The different exons of each transcript are represented by dark and light blue boxes. The RNG/MRC probes are represented on the first line; the light green box indicates the ‘current optimal probe’. The red box indicates the RNG/MRC probe(s). The blue box indicates a probe selected for a local microarray production. Each set of probes is represented on a distinct line. Affymetrix probe sets are represented by their first and last 25-mer perfect match probes. Additional information about the transcript or probes, such as gene chromosomal location, probe specificity, etc. are provided as clickable links. Subforms provide information about Gene Ontology annotations, bibliographic references or tissue-specificity.

Definition of the X_HYBRID specificity score. Typical picture of a probe specificity analysis, as available from the MEDIANTE interface (). Each column represents the number of BLAST hits in the MEDIANTE database (blue), Ensembl database (green), RefSeq database (red) for the BLAST expect-value indicated at bottom. The X_HYBRID score for a probe was calculated as the maximal x_hybrid scores among the three databases. Based on expect-values equal to 10, 1, 10⁻¹, 10⁻², 10⁻³, 10⁻⁴, 10⁻¹⁵, 10⁻²⁰, a ‘rank’ was defined, ranging from 0 for an expect-value equal to 10 to 6 for an expect-value equal to 10⁻¹⁵. For instance, the oligonucleotide depicted in has an extra hit in RefSeq for an expect-value of 10⁻¹, thus defining a rank equal to 2. The number of extra hits between the rank and the lowest expect-value is called delta (Δ). In the example shown in , Δ is equal to 1. Δ is always kept in the interval from 1 to 9, meaning that when there are more than 9 extra hits, Δ is kept to 9. A x_hybrid score is defined for each BLAST database (i.e. MEDIANTE, RefSeq, Ensembl) as a decimal number, where the integer part corresponds to the rank, and the leftovers to Δ. The final X_HYBRID score for a probe is defined as the maximal x_hybrid score obtained against the 3 BLAST databases.