PMC

A: Transmission electron micrographs of HepG2 cells from the control group( EM , original magnification × 6200); B: Transmission electron micrographs of HepG2 cells from the αIR3 group (EM, original magnification × 6200).

A: Immunohistochemical findings of IGF-IR in Chang liver cells. Chang liver cells showing positive-staining of IGF-IR in the cell membranes of the cells (SABC, original magnification × 100); B: Immunohistochemical findings of IGF-IR in HepG2 cells. HepG2 cells showing stronger positive-staining of IGF-IR in the cell membranes of the cells than that of Chang liver cells (SABC, original magnification× 100).

Effects of αIR3 on in vitro growth of HepG2 cells treated with various concentrations of αIR3 for different periods of time. αIR3 of 0.1 μg/mL could stimulate HepG2 cells to proliferate, while αIR3 inhibited HepG2 cell proliferation in a dose- and time-dependent manner at a concentration ranging from 0.2 μg/mL to 4.0 μg/mL.