PMC

LPS-stimulated IL-6 and CCL2 secretion of T1D and control monocytes. Monocytes from 10 controls and 10 T1D patients were cultivated as described in the text. IL-6 (A) and CCL2 (C) were determined in the supernatant of cells treated with 1 μg/ml LPS for 24 h. CCL2 was also determined in unstimulated monocytes (B). Outliers are indicated by the dots.

SOD 2 in LPS-stimulated T1D and control monocytes. Monocytes from four different controls (C1 to C4) and four different T1D patients (D1 to D4) were cultivated with or without LPS for 24 h. SOD 2 was determined by immunoblot and a representative immunoblot from control 1 and 2 (C1, C2) and T1D patient 1 and 2 (D1, D2) is shown.

LPS-mediated CXCL8 and Apo E in T1D and control monocytes. Monocytes from controls and T1D patients were cultivated with or without LPS for 24 h. CXCL8 was determined in non-activated monocytes of controls and T1D patients (A) and LPS-activated cells (B). Intracellular Apo E was analyzed by immunoblot and the result for monocytes from control 1 and 2 (C1, C2) and T1D patient 1 and 2 (D1, D2) is shown (C). Apo E was determined in the supernatants of 10 control and 10 T1D monocytes treated with 1 μg/ml LPS for 24 h. Outliers are indicated by the dots (D).