PMC

In situ hybridization for EBER1 in gastric carcinoma tissue. A: In situ EBER1 hybridization with antisense probes. Strong signals were observed in the nuclei of all tumor cells; B: In situ EBER1 hybridization with sense probes; C: Hematoxylin/eosin (H&E) staining in the adjacent pair of EBER1 in situ hybridization. original magnification ×200.

RT-PCR and Southern hybridization analysis of EBNA gene transcription using promoters. M: DIG-labeled DNA molecular weight marker VIII (Roche). Lane 1: EBV-positive LCL(positive control); lane 2: EBV-negative Ramos cells(negative control); lanes 3-13: EBV-positive gastric carcinoma samples. GAPDH mRNA was amplified to check pertinent RNA extraction and results were shown by EB staining.

Detection of EBV latent gene expression (A) and EBV lytic gene expression (B) by RT-PCR and Southern hybridization. M: DIG-labeled DNA molecular weight marker VIII (Roche). Lane 1: EBV-positive LCL (positive control); lane 2: EBV-negative Ramos cells (negative control); lanes 3-13: EBV-positive gastric carcinoma samples. GAPDH mRNA was amplified to check pertinent RNA extraction and results were shown by EB staining.