PMC

The structure comparison of the tocopherols A) with troglitazone B).

Tocopherol upregulation of PPARγ protein by Western Blot analysis of A) 24-hour tocopherol-treated SW480 nuclear extracts: lane 1 – 10 μM γ-tocopherol, lane 2 – 5 μM γ-tocopherol lane 3 – 10 μM α-tocopherol, lane 4 – 5 μM α-tocopherol, lane 5 – 100 μM troglitazone, lane 6 – blank, lane 7-ethanol carrier control.B) Bar graph of densitometries for Western Blot shown in figure .

Tocopherol upregulation of PPARγ mRNA expression detected by QPCR after 24 hours of treatment with 5 μM tocopherol. A) Relative copy number of a representative QPCR AT 5 μM tocopherol concentration. B) Fold increase data of the representative QPCR data shown in figure . C) Average fold increase data of three independent experiments at 5 μM tocopherol concentration. * shows sample statistical difference compared to the vehicle-treated.

Tocopherol upregulation of PPARγ mRNA expression detected by QPCR after 24 hours of treatment with 10 μM tocopherol. A) Relative copy number of a representative QPCR AT 10 μM tocopherol concentration. B) Fold increase data of the representative QPCR data shown in figure . C) Average fold increase data of three independent experiments at 10 μM tocopherol concentration. * shows sample statistical difference compared to the vehicle-treated.

Tocopherol upregulation of PPARγ protein by Western Blot analysis of A)48-hour tocopherol-treated SW480 whole cell lysates: lane 1 – PPARγ control from transfected COS cells, lane 2 – negative control from untreated Jurkat cells, lane 3 – vehicle-treated control, lane 4 – 10 μM α-tocopherol, lane 5 – 10 μM γ-tocopherol, lane 6 – 100 μM troglitazone. B) Bar graph of densitometries for Western Blot shown in figure .