Normal differentiation of ILK-deficient chondrocytes. In situ hybridization of growth plate from control (fl/+, Cre, A, C, E, and G) or mutant (fl/fl, Cre, B, D, F, and H) E16.5 mouse femur. (A and B) Collagen type II (col II), antisense probe. (C and D) Collagen type X (col X), antisense probe. (E and F) Ihh, antisense probe. (G and H) PTHR1, antisense probe. The zone of expression is bracketed in E–H. Note that these markers of differentiation exhibit the same pattern of expression in control and mutant littermates. Background staining with sense probes was undetectable (not depicted). Bars, 100 μm.