PMC

Surfactin inhibits biofilm formation on urethral catheters. The indicated organisms were grown overnight at 30°C in sterile urethral catheters containing medium with and without 100 μg of surfactin as described in the text. Biofilms were analyzed by staining with crystal violet.

Surfactin inhibits biofilm formation by wild-type S. enterica. PVC wells were coated with the indicated amounts of surfactin before inoculation with S. enterica. After overnight incubation at 30°C, the wells were rinsed out and stained with crystal violet. The biofilm is concentrated at the interface between the air and the liquid medium (indicated by arrows).

Biofilm formation by S. marcescens and its mutants. Wild-type S. marcescens 274 and its serrawettin mutants (SMu1a, SMu4e, SMu13a, and SMu4853b []) were analyzed for biofilm formation in the absence and presence of surfactin as described in the legend to Fig. . The OD₆₃₀ of all the cultures was ≈0.15 (not shown). Open bars, no surfactin; shaded bars, 100 μg of surfactin.

Kinetics of biofilm formation by wild-type S. enterica. SJW1103 was cultured at 30°C in PVC 96-well plates containing LB without NaCl plus 0.2% glucose. Samples were harvested at designated time points to determine growth (OD₆₃₀) and biofilm formation after crystal violet staining (OD₅₅₀) as described in the text. ▪, OD₆₃₀; ●, OD₅₅₀.

Biofilm formation by S. enterica swarming mutants. Growth conditions were as described in the legend to Fig. . The OD₆₃₀ of all samples was approximately 0.2. Arrow points to biofilm levels (OD₅₅₀) of wild-type SJW1103, and arrowheads point to those of mutants not affected in swarming. The data are means of triplicate samples monitored on the same day. There was a significant variation in the absolute biofilm values of samples processed on different days, but the relative values with respect to the wild-type value were very reproducible.

Addition of surfactin and other surfactants to a preformed biofilm accelerates biofilm dispersal. After S. enterica had reached an OD₆₃₀ of ≈0.15 to 0.2, the indicated surfactants were gently mixed into the cultures in microtiter wells. Samples were harvested, and either growth (A), as determined by OD₆₃₀, or biofilm levels (B), as measured by OD₅₅₀ of crystal violet-stained material, were analyzed at the indicated time points. ⧫, no treatment; ○, 100 μg of surfactin; ×, 0.25% Tween 80; ▪, 100 μg of rhamnolipid; ▴, 0.2% SDS. The data are means of triplicate samples monitored on the same day.