Anion-exchange HPLC profiles of wild-type GST-UL97 enzyme reactions at 8 h with 3H-labeled GCV (A) or 3H-labeled ACV (B), as described in the Materials and Methods section. Retention times of authentic carriers which appear in the UV profile are as follows: ACV, 1.8 min; GCV, 1.8 min; AMP, 12.2 min; GCV-MP, 14.3 min; ACV-MP, 15.9 min; GMP, 17.1 min; ADP, 20.4 min; ATP, 25.0 min; and GTP, 27.2 min. The UV peak at 19.5 min is an unknown peak generated from the UL97 preparation. Radioactive peaks were collected as 1-ml fractions. The scale for the counts per minute (cpm) was expanded to more clearly show the MP cpm; the cpm in fraction 2 were approximately 600,000, and those in fraction 3 were approximately 80,000.