PMC

TSP-1 mRNA expression in mock-infected (−) and AD169-infected HFF (+) cells without treatment, after treatment with GCV or after treatment with ISIS 2922. Reverse transcription PCR was done using specific primers for TSP-1 and GAPDH (standard control). Products were visualized by staining with silver nitrate on polyacrylamide gel. The bands were analyzed by densitometry (A). Relative density values of TSP-1 mRNA are depicted as the mean of three experiments ± SD (B).

Time course of TSP-1 mRNA expression in mock-infected (−) and AD169-infected (+) HFF cells. Total RNA was isolated 4, 24, or 72 hours p.i. Reverse transcription PCR was done using specific primers for TSP-1 and GAPDH, and products were visualized by staining with silver nitrate on polyacrylamide gel (A). The bands were analyzed by densitometry. Relative density values of TSP-1 mRNA are depicted as the mean of three experiments ± SD (B).

Time course of p53 protein expression in HFF (A) and U373MG (B) cells. p53 was measured by flow cytometry in mock-infected or AD169- or Hi91-infected cells 4, 24, and 72 hours p.i. The results are expressed as relative mean fluorescence intensity units. Data are depicted as means ± SD from one representative experiment performed in triplicate. Statistically significant differences were found between virus-infected and mock-infected HFF cultures (P < 0.05) after 24 and 72 hours as determined by Student’s t-test.

Time course of TSP-1 protein expression in HFF (A) and U373MG (B) cells. TSP-1 was measured by flow cytometry in mock-infected or AD169- or Hi91-infected cells 4, 24, and 72 hours after infection p.i. The results are expressed as relative mean fluorescence intensity units. Data are depicted as means ± SD from one representative experiment performed in triplicate. Statistically significant differences were found between virus-infected and mock-infected cultures (P < 0.05) after 24 and 72 hours as determined by Student’s t-test.

Effects of antiviral drugs GCV and ISIS 2922 on HCMV IEA (•) and LA (▾) expression in HFF cultures (A and C) and U373MG cultures (B and D) infected with AD169. The numbers of HCMV IEA- and LA-positive cells were determined by means of immunoperoxidase staining and counted 24 hours (IEA) and 72 hours (LA) p.i. The results are expressed as percentage of IEA/LA-positive cells of infected controls without drug treatment. In control cultures, more than 98% and 92% of cells stained positive for IEA or LA, respectively. Data are shown representative of three independent experiments.