Brucella and Its Hidden Flagellar System

Brucella, a Gram-negative bacterium with a high infective capacity and a wide spectrum of hosts in the animal world, is found in terrestrial and marine mammals, as well as amphibians. This broad spectrum of hosts is closely related to the non-classical virulence factors that allow this pathogen to establish its replicative niche, colonizing epithelial and immune system cells, evading the host’s defenses and defensive response. While motility is the primary role of the flagellum in most bacteria, in Brucella, the flagellum is involved in virulence, infectivity, cell growth, and biofilm formation, all of which are very important facts in a bacterium that to date has been described as a non-motile organism. Evidence of the expression of these flagellar proteins that are present in Brucella makes it possible to hypothesize certain evolutionary aspects as to where a free-living bacterium eventually acquired genetic material from environmental microorganisms, including flagellar genes, conferring on it the ability to reach other hosts (mammals), and, under selective pressure from the environment, can express these genes, helping it to evade the immune response. This review summarizes relevant aspects of the presence of flagellar proteins and puts into context their relevance in certain functions associated with the infective process. The study of these flagellar genes gives the genus Brucella a very high infectious versatility, placing it among the main organisms in urgent need of study, as it is linked to human health by direct contact with farm animals and by eventual transmission to the general population, where flagellar genes and proteins are of great relevance.


Introduction
Brucella abortus is a Gram-negative, non-motile, coccobacillary bacterium and a facultative intracellular pathogen, causing disease in both animals and humans [1]. Brucellosis, the name given to the disease caused by this pathogen, is a zoonosis described in cattle and other mammals. Being an opportunistic agent, it can infect humans, and diagnosis is difficult [2]. This disease can be characterized by its ability to become a chronic infection, which in bovines can cause abortions, stillbirths, the birth of weak calves, and sterility in males [3]. It is endemic in many areas of the world, and, in humans, the main sources of infection are through the consumption of contaminated meat and dairy products and through contact with secretions from infected animals [2,4,5]. Brucella enters the host organism through traumatized skin (wounds) or through the air to the mucosa in the form of an aerosol [6]. Once inside the host organism, Brucella spreads and multiplies in the lymph nodes, spleen, liver, bone marrow, mammary glands, and sexual organs via macrophages [7]. Brucella can compromise the gastrointestinal, hepatobiliary, genitourinary, skeletal muscle, cardiovascular system, and integumentary systems [8], which can become a chronic condition, crossing the blood-brain barrier through infected monocytes like a Trojan horse and achieving re-infection in the midbrain, causing neurobrucellosis [9]. If the bacteria are not killed, they can survive for long periods inside these phagocytic cells, in phagosomes, where they can multiply by inhibiting fusion with the lysosome through rapid acidification into The correct functioning and conformation of this bacterial organelle depend on the perfect arrangement of the proteins that form the MS-ring, the transporter channel proteins, and the C-ring. Once the base of the flagellum has formed on the membrane, FlgJ is cleaved from the basal body. (C) This correct arrangement of previous proteins makes it possible to reach the end of the flagellum formation given by the cluster conglomerate of rod proteins, the LP-ring, followed by the hook, and finally, by the formation of the filament (FliC) that mediates the movement allowed by proton pumping from the periplasm. In Gram-negative bacteria, the final protein that "seals" the filament is called CapD (FliD); however, this has not yet been described in Brucella, even though Brucella presents the gene for its expression. LPS: lipopolysaccharide; OMP: outer membrane protein; IMP: inner membrane protein.
In Brucella, it has been suggested that certain proteins participate in this regulatory cascade of flagellar genes, with certain genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1, and RpoE2, where the deletion of the rpoE1 gene increases the production of the flagellar protein FlgE [57]. In addition, this mutation also increases the gene expression of fliF, flgE, fliC, flaF, and flbT (Table 1), where the expression of these genes is also controlled by the master regulator ftcR, which is mediated by the rpoE1 gene product and other, as yet unknown, proteins [62]. The correct functioning and conformation of this bacterial organelle depend on the perfect arrangement of the proteins that form the MS-ring, the transporter channel proteins, and the C-ring. Once the base of the flagellum has formed on the membrane, FlgJ is cleaved from the basal body. (C) This correct arrangement of previous proteins makes it possible to reach the end of the flagellum formation given by the cluster conglomerate of rod proteins, the LP-ring, followed by the hook, and finally, by the formation of the filament (FliC) that mediates the movement allowed by proton pumping from the periplasm. In Gram-negative bacteria, the final protein that "seals" the filament is called CapD (FliD); however, this has not yet been described in Brucella, even though Brucella presents the gene for its expression. LPS: lipopolysaccharide; OMP: outer membrane protein; IMP: inner membrane protein.
In Brucella, it has been suggested that certain proteins participate in this regulatory cascade of flagellar genes, with certain genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1, and RpoE2, where the deletion of the rpoE1 gene increases the production of the flagellar protein FlgE [57]. In addition, this mutation also increases the gene expression of fliF, flgE, fliC, flaF, and flbT (Table 1), where the expression of these genes is also controlled by the master regulator ftcR, which is mediated by the rpoE1 gene product and other, as yet unknown, proteins [62]. Moreover, the mutation of rpoE1 increases the promoter activity of the flagellar master regulator ftcR, suggesting that the protein group with RpoE1 acts upstream of ftcR, repressing its expression. FtcR is a flagellar two-component regulator described in Brucella melitensis, which is involved in the expression of class II genes, flgE (hook proteins) and fliF (basal body proteins) [57,68], as well as certain proteins that regulate the expression of the class-III gene, such as FlaF, which represses FliC, and FlbT ( Figure 2A). In addition to being a self-regulating protein, FlbT enables the production of the FliC protein, forming part of the expression of class III genes [70]. FliC production in Brucella melitensis is not subject to the basal body and hook termination, unlike E. coli and other bacteria [71,72]. In B. melitensis, the mutant for FlgE and FliF still produces flagellin, as previously indicated; therefore, the expression of FliC requires the FlbT regulator for its expression [71]. Genes have been shown to be involved in quorum sensing (QS) in Brucella; vjbR and blxR work as transcriptional regulators involved in the virulence of Brucella and participate in the formation of biofilms through the expression of flagellar genes [73]. VjbR is required by B. melitensis for transcription of the type-IV secretion system and the expression of several flagellar genes (fliF, flhA, motB, or flgE) that contribute to its virulence in mice, where ftcR is partially activated by VjbR [46,74] (Figure 2B). New studies have identified that FliC production is not controlled solely by the master regulator, FlbT. Along with the abovementioned components, we must mention YbeY endoribonuclease, one of the best-conserved enzymes in different organisms, as it is related to an important variety of metabolic activities including, for example, proper cell morphology, mRNA transcription levels, and virulence, as well as indirectly participating in the expression of certain flagellar proteins in B. abortus [75,76]. When the ybeY gene was mutated in Brucella abortus, it was found that the product of this gene participates indirectly in the gene expression of transcriptional regulatory proteins, since ftcR mRNA levels were elevated in the mutant strain. This is linked to the expression of FliC (flagellin), which is also significantly elevated in the ∆ybeY strain [77], and, like FtcR, is required for FliC production. The increase observed in the expression of fliC mRNA in the ∆ybeY strain could be due to the increase in FtcR levels ( Figure 2C) [46,68]. transcriptional regulatory proteins, since ftcR mRNA levels were elevated in the mutant strain. This is linked to the expression of FliC (flagellin), which is also significantly elevated in the ΔybeY strain [77], and, like FtcR, is required for FliC production. The increase observed in the expression of fliC mRNA in the ΔybeY strain could be due to the increase in FtcR levels ( Figure 2C) [46,68].  From the above information and added to the context of Brucella in the literature, it can be stated that the action of flagellar genes is not only subject to the expression of master regulators but is also related to the expression of other genes belonging to other bacterial metabolic cascades. This is clear from different studies conducted on mutant strains of different genes in Brucella (Table 2). ∆aibP (QS gen) B. melitensis Expression of fliC, fliF, flgE y flbT was significantly downregulated, with enhanced virB genes expression and VirB8 production. [63] ∆BM-LOV-HK (light-sensing histidine kinase)

Flagellar Proteins as Virulence Factors in Brucella spp.
Virulence factors are molecules produced by pathogens that favor their infective capacity, allowing them to colonize host tissue, evade the host immune response, enter and exit cells, or acquire nutrients (e.g., iron). These are key elements in the adaptation and survival of pathogens in the host organism [84]. The most important characteristic of Brucella is the ability to survive and multiply within both phagocytic and non-phagocytic cells [85]. There is also evidence that certain proteins that make up part of the flagellum participate in mechanisms that allow the bacterium to establish its replicative niche and its infective chronicity in the host [57,63,64,68,69]. These flagellar virulence factors have even been studied as immunogenic agents in murine models for the development of brucellosis vaccines [67]. Bacteria of the genus Brucella have been described for some time as non-motile organisms [1]; however, certain findings reveal the presence of operons that contain flagellar genes [86], and, in certain species of Brucella, under special conditions-either cultivational or environmental-functional bacterial flagella are present [46,87]. For the induction of the disease, as indicated previously, Brucella must adhere, invade, and survive within mammalian cells. As previously mentioned, the flagellar genes of B. abortus, present in its genome, are an interesting finding in relation to a bacterium described as non-motile [86]. This discovery may indicate that these proteins are involved in other vital functions for the establishment of bacterial infection, as in the case of the FlgJ protein, which, when encoded by the open reading frame BAB1_0260, participating in virulence and being mutated, limits the ability to form biofilms in vitro [69,88]. In E. coli, Salmonella, and other bacterial groups, this flagellar conformation and its subsequent formation begin with the action of the FlgJ protein. FlgJ is the rod cap protein most closely related to the early stages of flagellum formation and rod assembly; once the latter is formed, FlgJ is cleaved from the emerging flagellum and is essential for its genesis since it has dual canonical activity [89], hydrolysis on the PG and scaffold activity, enabling the assembly of the initial units of the flagellum in the periplasmic space [90]. It has been shown in S. enterica that the FlgJ muramidase plays an important role in flagellum synthesis, being secreted through the type-III flagellar export system and exhibiting its enzymatic activity in the C-terminal end [89,91]. This domain, of approximately 164 amino acids in length, presents two important catalytic residues for this muramidase activity. This is given by glutamic acid (E233) and aspartic acid (D248), the function of which is to make a hole in the PG, thus allowing the penetration of the shaft through the periplasmic space so that the hook and the filament can later assemble in the extracellular space [91]. It has been reported, however, that in S. enterica, mutants for flgJ maintain the ability to swim after long incubation times, which indicates that muramidase activity is not entirely necessary for flagellum formation, suggesting that flagellum formation is due to the assembly upon biogenesis of new membranes and new PG [92]. Likewise, in Rhodobacter sphaeroides, SltF is present, a muramidase associated with the flagellar system. Unlike the FlgJ of S. enterica, it is transported to the periplasm by the Sec translocase pathway, with the ability to produce a hole in the PG layer for the subsequent penetration of the flagellar structure [93,94]. In the case of Caulobacter crescentus, a bacterium that has a cell cycle very similar to Brucella [95], it possesses PleA, an enzyme homologous to lytic transglycosylase. This protein contains a region that is similar to a peptidoglycan-hydrolytic active site; a point mutation at this site in PleA results in the loss of the flagellum and pilus biogenesis. Furthermore, PleA is required for flagellar assembly, indicating its involvement during flagellum formation [96]. B. abortus has a flagellar protein with hydrolase activity on peptidoglycan, the FlgJ protein (BAB1_0260) located in genomic island three (GI-3) (https://www.ncbi.nlm.nih.gov/protein/82615263, accessed on 15 November 2021), which may be involved in the virulence of this species [69]. Although the muramidase activity has not yet been studied in Brucella, its activity in that part of the PG is doubtful; however, the bioinformatic bases suggest an important role for FlgJ in the initial stages of flagellar genesis, due to its glucosaminidase activity (https: //www.kegg.jp/dbget-bin/www_bget?bmf:BAB1_0260, accessed on 15 November 2021). A comparative analysis of the amino acid sequence encoding FlgJ from Brucella with other bacteria reveals that the FlgJ from Brucella does not have the typical conserved sequences of FlgJ with muramidase activity found in E. coli and S. enterica (Figure 3). Even so, according to some databases, FlgJ from Brucella presents activity on the PG (acetylglucosaminidase activity). Like other FlgJ from other bacteria, it could be hypothesized that an extra element might be participating in this muramidase activity, to make a hole in the peptidoglycan before the assembly of proteins in the flagellum. This occurs in Rhodobacter sphaeroides or Caulobacter crescentus, as mentioned above, where other PG lytic enzymes would participate, together with the FlgJ from Brucella. This would, therefore, enable the correct assembly of the base of the flagella in the bacterial membrane. By obtaining mutants for the BAB1_0260 in B. abortus, it was possible to evaluate whether FlgJ was involved in pathogenicity as a virulence factor, showing that its deletion is not lethal to bacterial survival [69,97,98]. The FlgJ hydrolyzing activity on peptidoglycan (PG) plays an important role in the growth of this bacterium, specifically during the remodeling of PG during its cell division, which would explain why the elimination of the flgJ gene significantly reduces its growth. The bacterial flagellum is associated with functions that differ among bacteria. In addition to motility, it may be related to adherence and biofilm formation [99,100], processes closely related to the chronicity of the infections caused by different bacterial groups [101,102]. Biofilms are extracellular polymeric elements (EPS) produced by microorganisms, which include polysaccharides, proteins, nucleic acids, and lipids, which enable their adhesion to various surfaces and the interaction among bacteria (cell communication, competition, cooperation, or the horizontal movement of genes) [103]. The molecular mechanisms of how Brucella generates biofilms and the participating elements have scarcely been explored, since their replicative niche is cells in living tissue, which makes their study in this area difficult. This finding shows that FlgJ participates in the adherence or secretion of proteins involved in the process, making it clear that in the formation of biomass associated with B abortus 2308 biofilms, FlgJ is directly related to the levels of bacterial growth and division [69]. This suggests that reduced bacterial adherence decreases the colonization of host tissues, where several factors are involved, including the flagellum [104]. Likewise, microarray assay analyses have found that the effect of erythritol on the expression of B. melitensis genes (cultivated with or without erythritol) was shown to be the upregulation of two main virulence pathways, in response to erythritol exposure-the VirB type IV secretion system and flagellar proteins (the third-largest cluster of orthologous groups), suggesting a role for erythritol in virulence [82].
Microorganisms 2022, 10, x FOR PEER REVIEW 9 of 18 polysaccharides, proteins, nucleic acids, and lipids, which enable their adhesion to various surfaces and the interaction among bacteria (cell communication, competition, cooperation, or the horizontal movement of genes) [103]. The molecular mechanisms of how Brucella generates biofilms and the participating elements have scarcely been explored, since their replicative niche is cells in living tissue, which makes their study in this area difficult. This finding shows that FlgJ participates in the adherence or secretion of proteins involved in the process, making it clear that in the formation of biomass associated with B abortus 2308 biofilms, FlgJ is directly related to the levels of bacterial growth and division [69]. This suggests that reduced bacterial adherence decreases the colonization of host tissues, where several factors are involved, including the flagellum [104]. Likewise, microarray assay analyses have found that the effect of erythritol on the expression of B. melitensis genes (cultivated with or without erythritol) was shown to be the upregulation of two main virulence pathways, in response to erythritol exposure-the VirB type IV secretion system and flagellar proteins (the third-largest cluster of orthologous groups), suggesting a role for erythritol in virulence [82].

Evolutionary Aspects of Brucella and Flagellar Genes
One of the orders that fall within the class of Alpha-proteobacteria is the order of the Rhizobiales; among the families of this order are the Brucellaceae, with genera such as Mycoplana, Ochrobactrum and Brucella, and the family Rhizobiaceae, which includes the genera Allorhizobium, Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium. The genus Brucella developed as intracellular animal pathogens, while the genus Rhizobium is associated with soil and forms nitrogen-fixing nodules but, according to this evolutionary background, they have similar evolutionary characteristics [105]. Some evidence to hypothesize an evolutionary relationship between Brucellaceae and Rhizobiaceae is given by the presence of certain genes that they have in common; for example, some genes involved in symbiosis in Sinorhizobium meliloti are homologous to genes involved in Brucella pathogenesis [106]. The bacA gene that is involved in the symbiosis of Sinorhizobium meliloti has its homologous gene in Brucella abortus, which plays a role in the survival of Brucella in macrophages and in its pathogenesis in mice [106,107]. Along with the above findings and in relation to the bacterial flagellum, we should mention that Bradyrhizobium diazoefficiens, the nitrogen-fixing symbiont of soybean, possesses two flagellar systems that evolved independently [105,108,109]. This dual flagellar system, present in such bacteria, performs its function as a single flagellum, which is similar to the polar flagellum of Brucella spp. [109,110]. A comparative analysis of the genetic sequences encoding the FliC protein of Brucella abortus 2308 flagella revealed that bacteria of different

Evolutionary Aspects of Brucella and Flagellar Genes
One of the orders that fall within the class of Alpha-proteobacteria is the order of the Rhizobiales; among the families of this order are the Brucellaceae, with genera such as Mycoplana, Ochrobactrum and Brucella, and the family Rhizobiaceae, which includes the genera Allorhizobium, Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium. The genus Brucella developed as intracellular animal pathogens, while the genus Rhizobium is associated with soil and forms nitrogen-fixing nodules but, according to this evolutionary background, they have similar evolutionary characteristics [105]. Some evidence to hypothesize an evolutionary relationship between Brucellaceae and Rhizobiaceae is given by the presence of certain genes that they have in common; for example, some genes involved in symbiosis in Sinorhizobium meliloti are homologous to genes involved in Brucella pathogenesis [106]. The bacA gene that is involved in the symbiosis of Sinorhizobium meliloti has its homologous gene in Brucella abortus, which plays a role in the survival of Brucella in macrophages and in its pathogenesis in mice [106,107]. Along with the above findings and in relation to the bacterial flagellum, we should mention that Bradyrhizobium diazoefficiens, the nitrogen-fixing symbiont of soybean, possesses two flagellar systems that evolved independently [105,108,109]. This dual flagellar system, present in such bacteria, performs its function as a single flagellum, which is similar to the polar flagellum of Brucella spp. [109,110]. A comparative analysis of the genetic sequences encoding the FliC protein of Brucella abortus 2308 flagella revealed that bacteria of different genera in the order Rhizobiales show high homology of sequences and structural domains of the FliC protein ( Figure 4). This is in conjunction with the consideration that a high percentage of bacteria of the Rhizobiales order are considered to be environmental microorganisms and present bacterial flagella [111][112][113].
Microorganisms 2022, 10, x FOR PEER REVIEW 10 of 18 genera in the order Rhizobiales show high homology of sequences and structural domains of the FliC protein ( Figure 4). This is in conjunction with the consideration that a high percentage of bacteria of the Rhizobiales order are considered to be environmental microorganisms and present bacterial flagella [111][112][113].  Thus, we could speculate that Brucella diverged from common ancestors that are generally associated with soil, which are plant symbionts; that, like Brucella, they are intracellular and, therefore, do not require the expression of flagella. However, if the genes that encode the vast majority of the constituent proteins of a flagellum are subjected to selective pressure from their environment, the flagellum can be expressed [46,108]. Furthermore, all this is closely related to the proposition that Brucella's ancestor was probably a free-living plan-related bacterium, possessing one chromosome and evolving into an animal parasite with two chromosomes [114] (Figure 5). Thus, we could speculate that Brucella diverged from common ancestors that are generally associated with soil, which are plant symbionts; that, like Brucella, they are intracellular and, therefore, do not require the expression of flagella. However, if the genes that encode the vast majority of the constituent proteins of a flagellum are subjected to selective pressure from their environment, the flagellum can be expressed [46,108]. Furthermore, all this is closely related to the proposition that Brucella's ancestor was probably a free-living plan-related bacterium, possessing one chromosome and evolving into an animal parasite with two chromosomes [114] (Figure 5).
Within this evolutionary process, Brucella has acquired different foreign DNA fragments by horizontal transfer, which are distributed throughout the genome, and which encode several proteins, mainly of unknown function [115]. Yet, among these genes, there are some that encode virulence factors, where some flagellar proteins that are present in certain Brucella ORFs are worthy of note [69,86,116,117]. Microorganisms 2022, 10, x FOR PEER REVIEW 11 of 18 Figure 5. (A) In terms of its evolutionary origin, it is postulated that Brucella may have been a freeliving bacterium. (B) Over time, and according to its evolution, it remained in contact with other microorganisms, such as soil bacteria and fungi, managing to acquire certain genes that, therefore, improved its metabolic resources. (C) Environmental changes helped give it the ability to adapt to new hosts, such as eukaryotic cells. In Brucella, certain virulence factors that are different from other bacteria, like its LPS, its type-four secretion system (T4SS) and the BvrR/BvrS system, enable Brucella to interact with the host cell surface and form an early Brucella-containing vacuole (BCV) for its subsequent interaction with the endoplasmic reticulum (ER), where bacteria multiply and reach their replicative niche. Likewise, the presence of flagellar genes and the active participation of flagellum proteins in different functions, such as the translocation of proteins to the outside and the formation of adhered biofilm-associated biomass gave Brucella the ability to be a successful organism in achieving its infectious chronicity and evading the immune response of the host organism.
Within this evolutionary process, Brucella has acquired different foreign DNA fragments by horizontal transfer, which are distributed throughout the genome, and which encode several proteins, mainly of unknown function [115]. Yet, among these genes, there are some that encode virulence factors, where some flagellar proteins that are present in certain Brucella ORFs are worthy of note [69,86,116,117].

Flagellum and Immune Response against Brucella
The function of flagella as bacterial nanomachines is to mediate motility. The flagellar filament, composed of a single protein, flagellin, is assembled by the basal body via a pathway homologous to the type 3 secretion system (T3SS) [118]. In motile bacteria, such as Bartonella species (lineages 1-3, except Bartonella bovis), flagella are thought to mediate erythrocyte internalization as a mechanical force or as adhesion molecules [119]. The Bartonella species of lineage 4 that are non-motile, due to the loss of flagella, mediate interaction with erythrocytes through Trw T4SS, suggesting that Trw has functionally replaced the flagella in establishing interaction with erythrocytes [120]. Brucella are non-motile bacteria but flagella genes are essential for their virulence [121]. Given that Brucella is also found in erythrocytes [122], we could speculate that there is a mechanism similar to the one mentioned earlier, where flagellar genes play a fundamental role in the survival and stealth of Brucella, even, one could say, in the evasion of the immune response. Brucella spp. evade detection through TLR4 by producing a poorly recognized form of lipid A. Brucella lipid A contains a much longer fatty-acid residue (C28) than enterobacterial LPS Figure 5. (A) In terms of its evolutionary origin, it is postulated that Brucella may have been a free-living bacterium. (B) Over time, and according to its evolution, it remained in contact with other microorganisms, such as soil bacteria and fungi, managing to acquire certain genes that, therefore, improved its metabolic resources. (C) Environmental changes helped give it the ability to adapt to new hosts, such as eukaryotic cells. In Brucella, certain virulence factors that are different from other bacteria, like its LPS, its type-four secretion system (T4SS) and the BvrR/BvrS system, enable Brucella to interact with the host cell surface and form an early Brucella-containing vacuole (BCV) for its subsequent interaction with the endoplasmic reticulum (ER), where bacteria multiply and reach their replicative niche. Likewise, the presence of flagellar genes and the active participation of flagellum proteins in different functions, such as the translocation of proteins to the outside and the formation of adhered biofilm-associated biomass gave Brucella the ability to be a successful organism in achieving its infectious chronicity and evading the immune response of the host organism.

Flagellum and Immune Response against Brucella
The function of flagella as bacterial nanomachines is to mediate motility. The flagellar filament, composed of a single protein, flagellin, is assembled by the basal body via a pathway homologous to the type 3 secretion system (T3SS) [118]. In motile bacteria, such as Bartonella species (lineages 1-3, except Bartonella bovis), flagella are thought to mediate erythrocyte internalization as a mechanical force or as adhesion molecules [119]. The Bartonella species of lineage 4 that are non-motile, due to the loss of flagella, mediate interaction with erythrocytes through Trw T4SS, suggesting that Trw has functionally replaced the flagella in establishing interaction with erythrocytes [120]. Brucella are non-motile bacteria but flagella genes are essential for their virulence [121]. Given that Brucella is also found in erythrocytes [122], we could speculate that there is a mechanism similar to the one mentioned earlier, where flagellar genes play a fundamental role in the survival and stealth of Brucella, even, one could say, in the evasion of the immune response. Brucella spp. evade detection through TLR4 by producing a poorly recognized form of lipid A. Brucella lipid A contains a much longer fatty-acid residue (C 28 ) than enterobacterial LPS (C 12 -C 16 ), and this modification greatly decreases its endotoxic properties by reducing TLR4 agonist activity and, thus, the host immune response [123]. In addition to TLR4 evasion, Brucella evades detection by TLR5 by producing a flagellin that lacks the TLR5 agonist domain [124]. As a result, Brucella flagellin is a poor inducer of TLR5-mediated inflammatory responses [123]. In addition to manipulating T cell-mediated immune responses, Brucella can also control T cell-independent immune responses [125]. Therefore, it is likely that Brucella induces partial and transient immunosuppression to establish a chronic infection [126,127]. Conversely, certain flagellar proteins have been examined for their possible immunogenic value as vaccine candidates. Five flagellar genes (BAB1_0260 (FlgJ); BAB2_0122 (FliN); BAB2_0150; BAB2_1086; BAB2_1093) were taken into consideration for their ability to induce humoral and cellular responses and to protect mice against B. abortus. FlgJ and FliN proved to be protective antigens that produced humoral and cellular responses in mice [128]. In addition to the above findings, studies with the FliC protein have shown it to be a new potential antigen candidate for the development of a subunit vaccine against Brucella [129,130].

Conclusions and Outlook
In recent years, many new species of Brucella have been discovered, mainly pathogens of marine mammals and others capable of infecting terrestrial mammals, in addition to flagellated species present in amphibians [86]. This complicates the execution of control programs since the recently characterized Brucella species have high genetic flexibility and many of these isolates are motile, fast-growing, able to survive in the soil, more resistant to unfavorable conditions of environmental acidity, and more able to adapt to new nonmammalian hosts, such as amphibians, rapidly adapting to their environment to broaden their host range [87]. As Brucella is a pathogen with different routes of infection, it can survive in and out of mammalian hosts for a long time, even under unfavorable conditions. Brucella is characterized as being a stealthy microbe that tends to chronicity, instead of causing an acute fatal infection, standing out for being a successful microorganism by evading the immune response and keeping its hosts alive to maintain its own survival. Flagellar biogenesis shows interesting variations among different proteobacteria and even among members of Alphaproteobacteria. The flagellar genes of Brucella are needed to establish infection in vivo in mice; however, the molecular basis of the impact of the flagellum on virulence in Brucella is still to be determined. This is evidenced by certain species of Brucella, such as B. melitensis 16M, B. ovis, B. abortus 2308, and certain Brucella spp. in amphibians expressing flagellar proteins and/or flagella at the intracellular level [46,64,68,69,87]. This is an event of great importance, considering that today there is a strong relationship between humans and farm animals, which will eventually lead to an increase in this zoonosis caused by Brucella. If we pay attention to the routes of infection that Brucella has, in relation to its scope in the mechanisms of pathogenesis and transmission of the infection, we may hypothesize its relationship to the possible expression of the flagellum during invasion, and its persistence in red blood cells in the murine model [122]. This phenomenon is closely related to the role that the flagellum could have during its residence inside blood-sucking insects [131]. With all the above, we could hypothesize that the flagellum is playing an important role in bacterial survival at the extracellular, intracellular, or environmental level. The study of the bacterial flagellum in a non-motile bacterium is undoubtedly a challenge that deserves attention since it seems to be directly involved in its survival in different hosts and in a hostile environment.
Some methodologies have been proposed in the literature to explain the importance of flagellar genetic sequences involved in the abovementioned events. The creation of mutants, the study of biofilm formation, and the infective capacity both in vitro and in vivo [69] are part of the most successful methodology for preliminary assays. Likewise, the study of flagellar protein translocation by different types of transporters, where the role of these proteins in Brucella virulence could be evaluated [132], might point out certain functional evidence between different bacterial structures. Flow cytometry analysis in infected cells, to discern the appearance of markers involved in the immune response [130], as well as immunoprecipitation assays to see eventual protein interactions [130], and simple experiments such as staining of the flagella at different growth times under a variety of conditions [66] would provide further data to clarify certain unknowns about flagellar function and structure. Furthermore, in the field of immunology, the preparation of vaccines of different flagellar proteins in search of potential immunogenic agents [133], and the use of bioinformatics tools for comparative study with other bacteria [134,135], with differing degrees of similarity in the mechanisms of infection are appropriate methodologies to gain more background information. This will allow us to understand more fully the structurefunction relationship of the flagellum in an organism described to date as a non-motile bacterium.