Figure 1

(A) Schematic of strategy used to stimulate J-Lat 5A8 cells with CD3/CD28 to promote HIV exit from latency

(B) Strategy to introduce human genome-wide mCherry-tagged shRNA library into J-Lat cells, and then stimulate cells with a 3 µg/ml CD3/CD28 to yield 15% double-positive cells. GFP and mCherry represent J-Lat 5A8 HIV-GFP, and shRNA expression, respectively.

(C) Calculations conducted on samples that are deep sequenced to obtain p-values to identify genes involved in HIV latency.

(D) FANCC, an example of a gene that promotes latency.

(E) CAPN10, an example of a gene that inhibits latency.

(F, G) Graphs depicting number of enriched (F) or disenriched (G) genes plotted as a function of signed log 10 P values.

See also Tables S1, S2 and S3