Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
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Cell Host Microbe. Author manuscript; available in PMC 2017 Dec 14.
Published in final edited form as:
Cell Host Microbe. 2016 Dec 14; 20(6): 785–797.
doi: 10.1016/j.chom.2016.11.001
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Figure 1

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High Complexity shRNA Screen to Identify Genes that Control HIV Latency

(A) Schematic of strategy used to stimulate J-Lat 5A8 cells with CD3/CD28 to promote HIV exit from latency

(B) Strategy to introduce human genome-wide mCherry-tagged shRNA library into J-Lat cells, and then stimulate cells with a 3 µg/ml CD3/CD28 to yield 15% double-positive cells. GFP and mCherry represent J-Lat 5A8 HIV-GFP, and shRNA expression, respectively.

(C) Calculations conducted on samples that are deep sequenced to obtain p-values to identify genes involved in HIV latency.

(D) FANCC, an example of a gene that promotes latency.

(E) CAPN10, an example of a gene that inhibits latency.

(F, G) Graphs depicting number of enriched (F) or disenriched (G) genes plotted as a function of signed log 10 P values.

See also Tables S1, S2 and S3

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